Biotin & Streptavidin Conjugates

Biotin ethylenediamine iodoacetamide is readily coupled to thiols.

Biotin ethylenediamine, hydrobromide (N-(5-(6-((biotinoyl)amino)hexanoyl)amino)pentylmaleimide) is identical to Neurobiotin™, a useful anterograde and transneuronal tracer.

Biotin-X free acid (6-((Biotinoyl)amino)hexanoic acid) can be easily converted to the mixed anhydride form, which is more reactive than the succinimidyl ester and thus can be used to react with aromatic amines or sterically hindered amines.

Biotin-XX free acid (6-((6-((Biotinoyl)amino)hexanoyl)amino)hexanoic acid) can be easily converted to the mixed anhydride form, which is more reactive than the succinimidyl ester.

Biotin-X succinimidyl ester (6-((Biotinoyl)amino)hexanoic acid succinimidyl ester) is readily coupled to a primary or secondary amine under mild conditions.

Biotin-XX succinimidyl ester (6-((6-((Biotinoyl)amino)hexanoyl)amino)hexanoic acid succinimidyl ester) is readily coupled to a primary or secondary amine under mild conditions. The longer XX arm facilitates the interaction between biotin and avidin or streptavidin.

Biotin SE (Biotin, succinimidyl ester) is readily coupled to a primary or secondary amine under mild conditions.

We developed biotin-rhodamine 110 as an alternative to biotin-4-fluorescein (90062) and fluorescein biotin (80019), both of which have been used for detection of biotin binding sites and the degree of biotinylation of proteins, and for the measurement of avidin and streptavidin in crude biological samples.

Fluorescein biotin (5-((N-(5-(N-(6-(Biotinoyl)amino)hexanoyl)amino)pentyl)thioureidyl) fluorescein) can be used to detect and quantify biotin binding sites and the degree of biotinylation of proteins. Also see biotin-rhodamine 110 (80022) and biotin-4-fluorescein (90062).

This fluorescent biotin derivative is a useful tracer for studying neuronal morphology. The biotin portion of the molecule allows researchers to amplify the fluorescent signal using standard biotin-avidin or biotin-streptavidin biochemistry.

Biotin-AM3-25 is a conjugate of AM3-25 and biotin. Membranes labeled with the probe can subsequently couple to avidin or streptavidin.

Biotin-11-dCTP (Biotin-11-2′-deoxycytidine-5′-triphosphate, tetralithium salt) can be enzymatically incorporated into DNA via nick translation, random priming, or 3′-end terminal labeling. The number 11 is the number of atoms in the linker between biotin and dCTP.

This product is similar to biotin-11-UTP (40033) and biotin-16-UTP (40023) except that it has a longer and water soluble spacer group, which should facilitate interaction between the biotin group and avidin or streptavidin.

Biotin-11-CTP (Biotin-11-cytidine-5′-triphosphate, tetralithium salt) can be enzymatically incorporated into RNA.

Biotin-11-UTP can be enzymatically incorporated into RNA. The number 11 is the number of atoms in the linker between biotin and UTP.

This product is similar to biotin-11-dUTP (40029) and biotin-16-dUTP (40022) except that it has a longer and water-soluble spacer group, which should facilitate interaction between the biotin group and avidin or streptavidin.