Fluorescent Proteins, Nucleotides & Other Conjugates

Biotin-X-C₅-maleimide (N-(biotinoyl)-N’-(iodoacetyl)ethylenediamine) is readily and selectively coupled to thiols.

Biotin ethylenediamine iodoacetamide is readily coupled to thiols.

Biotin ethylenediamine, hydrobromide (N-(5-(6-((biotinoyl)amino)hexanoyl)amino)pentylmaleimide) is identical to Neurobiotin™, a useful anterograde and transneuronal tracer.

Biocytin (ε-Biotinoyl-L-lysine) is a very useful cellular tracer that can be introduced into cells by microinjection.

Biotin-X free acid (6-((Biotinoyl)amino)hexanoic acid) can be easily converted to the mixed anhydride form, which is more reactive than the succinimidyl ester and thus can be used to react with aromatic amines or sterically hindered amines.

Biotin-XX free acid (6-((6-((Biotinoyl)amino)hexanoyl)amino)hexanoic acid) can be easily converted to the mixed anhydride form, which is more reactive than the succinimidyl ester.

Biotin-X succinimidyl ester (6-((Biotinoyl)amino)hexanoic acid succinimidyl ester) is readily coupled to a primary or secondary amine under mild conditions.

Biotin-XX succinimidyl ester (6-((6-((Biotinoyl)amino)hexanoyl)amino)hexanoic acid succinimidyl ester) is readily coupled to a primary or secondary amine under mild conditions. The longer XX arm facilitates the interaction between biotin and avidin or streptavidin.

Biotin SE (Biotin, succinimidyl ester) is readily coupled to a primary or secondary amine under mild conditions.

Biotium developed SDIP/Eu³ as an alternative to DPA/Tb³ (80104), which is used for vesicle fusion assays.

The principle of DPA/Tb³ for vesicle fusion assay is based on the fact that contact of the chelator dipicolinic acid (DPA) with terbium (III) forms an instant Tb³ -DPA complex that is ~10,000 times more fluorescent than free Tb³ . In the assay, separate vesicle populations are loaded with 2.5 mM TbCl3 in 50 mM sodium citrate, or 50 mM DPA in 20 mM NaCl.

Similar to other sulforhodamine dyes, the highly water-soluble sulforhodamine G can also be used as a polar tracer for the studies of cell morphology and neuronal cell-cell communication (1-3).

Similar to other sulforhodamine dyes, the highly water-soluble sulforhodamine B can also be used as a polar tracer for the studies of cell morphology and neuronal cell-cell communication (1-3). In addition, sulforhodamine B has been shown to be a superior protein stain for use in the quantification of cellular proteins of cultured cells.

Sulforhodamine 101 has a longer absorption wavelength than does sulforhodamine B. Like sulforhodamine B and G, in addition to their potential use in cancer drug screening, these fluorescent dyes have been primarily used as polar tracers for the studies of neuronal cell morphology and cell-cell communication.

RuBP-4S ([Ruthenium(bathophenanthroline)(bis(bathophenanthrolinedisulfonate))],disodium salt) is a luminescent ruthenium complex similar to RuBPS (80021).

Lucifer Yellow CH lithium salt (LY CH lithium salt) is a widely used polar molecular tracer for studying neuronal morphology. The fluorescent molecule contains a carbohydrazide that allow the molecule to be aldehyde-fixable.