Amine-Reactive Dyes
Succinimidyl esters (SE, also known as NHS esters) react with primary amines such as lysine residues in proteins. SE dyes are commonly used for labeling proteins and antibodies. The reaction requires basic pH for target amines to be reactive, and is usually performed in pH 8.3 sodium bicarbonate buffer.
We offer more than 25 colors of our bright and photostable CF® Dye SE and easy-to-use CF® Dye Labeling Kits, as well as a wide variety of other amine-reactive dyes and biotinylation reagents below. Biotium’s CF® Dye SE are more resistant to hydrolysis than other SE, resulting in excellent reaction efficiency.
DNP-dPEG4-NHS Ester
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A DNP moiety containing a PEG linker and an NHS ester terminal group which can be used for labeling biomolecules with a DNP hapten tag. The NHS ester will react with primary amines to form a stable amide bond while the hydrophilic PEG linker improves the solubility of the labeled bioconjugate.
MB Acid
MB (methylene blue) acid can be conjugated to peptides, proteins, drugs, polymeric materials and biomolecules with primary amine groups. The conjugate will have a blue color and be able to complex with nucleic acids.
CBQCA
CBQCA (3-(4-carboxybenzoyl)quinoline-2-carboxaldehyde) reacts specifically with primary amines to form conjugates that can be analyzed by electrophoretic or chromatographic methods. CBQCA also reacts with hydrophilic peptides and amino sugars.
Sulforhodamine 101-PEO12 SE
SR101-PEO12 SE (Sulforhodamine 101-PEO12-propionic acid succinimydyl ester; Texas Red®-PEO12 SE) has an approximately 47 Angstrom water-soluble PEG spacer that separates the dye from the amine-reactive SE group.
Sulforhodamine 101-PEO8 SE
SR101-PEO8 SE (Sulforhodamine 101-PEO8-propionic acid succinimydyl ester; Texas Red®-PEO8 SE) has an approximately 32 Angstrom water-soluble PEG spacer that separates the dye from the amine-reactive SE group.
Carboxyrhodamine 110-PEO4, SE, 5 isomer
5-CR110-PEO4 SE (full name: 5-Carboxyrhodamine 110-PEO4-propionate, succinimidyl ester, single isomer) is an excellent green fluorescent rhodamine dye for labeling amines. The dye has better solubility than the parent dye 5-CR110 SE and its long PEG spacer should reduce any steric effect of the dye on the molecule to be labeled.
Carboxyrhodamine 110 succinimidyl ester, 6-isomer
Amine-reactive form of single isomer carboxyrhodamine 110. For certain applications, the dye is preferred over the corresponding 6-carboxyfluorescein because of its exceptional photostability and fluorescence insensitivity to pH change (4-9).
Carboxyrhodamine 110 succinimidyl ester, 5-isomer
Amine-reactive form of single isomer carboxyrhodamine 110, useful in DNA sequencing. For many single-dye-labeling applications, the dye is preferred over other green fluorescent dyes including fluorescein and Alexa Fluor® 488 because of its exceptional photostability and fluorescence insensitivity to pH.
Carboxyrhodamine 110-X succinimidyl ester, 5-isomer
5-CR110-X, SE (full name: 5-Carboxyrhodamine 110-X, succinimidyl ester) is the amine-reactive form of 5-carboxyrhodamine 110 with a 6-carbon spacer.
TAMRA SE, 6 isomer in DMSO
6-TAMRA SE (full name: 6-Carboxytetramethylrhodamine succinimidyl ester) is the amine-reactive form of 6-TAMRA single isomer (90035).
ROX, SE, 6 isomer in DMSO
6-ROX, SE is the amine-reactive form of 6-ROX single isomer (90037) dissolved in anhydrous DMSO, suitable for labeling oligonucleotides.
SFX succinimidyl ester, 5 isomer
5-SFX has a 6-atom aminohexanoyl spacer group that separates the fluorescein dye and the amine-reactive group.
SFX succinimidyl ester, 6 isomer
6-SFX has a 6-atom aminohexanoyl spacer group that separates the fluorescein dye and the amine-reactive group.
ROX-PEO8 SE, 5 isomer
5-ROX-PEO8 SE (full name: 5-Carboxy-X-rhodamine-PEO8-propionate, succinimidyl ester) has an approximately 32 Angstrom water-soluble PEG spacer that separates the red fluorescent ROX dye and the amine-reactive SE group.
FAM-PEO8 SE, 5 isomer
5-FAM-PEO8 SE (full name: 5-Carboxyfluorescein-PEO8 propionate succinimidyl ester) has an approximately 32 Angstrom water-soluble PEG spacer that separates fluorescein and the amine-reactive SE group.
AMCA-X, SE
AMCA-X SE is an amine-reactive, UV-excitable, blue fluorescent dye.