Biotinylation Reagents
Biotin-PEO4-propionate SE
Biotin-PEO4-propionate SE (Biotin-PEO4-propionate succinimidyl ester) is a water soluble biotin derivative that can be readily coupled to primary or secondary amines.
Biotin Cadaverine, Trifluoroacetate Salt
Biotin-PEO3-amine
Biotin-PEO3-amine is a water soluble biotin derivative that can be readily coupled to activated carboxylic acids.
Biotin-4-fluorescein
Biotin-4-fluorescein (N-(5-aminopentyl)biotinamide, trifluoroacetic acid salt) has been reported to be a superior fluorescent biotin derivative for accurate measurement of avidin and streptavidin in crude biological samples. Binding of biotin to avidin and streptavidin is high affinity, fast and noncooperative.
Biocytin hydrazide
Biocytin hydrazide is an aldehyde-fixable cellular tracer that can be introduced into cells by microinjection. Biocytin hydrazide can also be used to label glycoproteins or other carbohydrate molecules (See Reactive Dyes for Labeling Glycoproteins and Other Carbohydrate Molecules).
Biotin-X cadaverine, trifluoroacetate salt
Biotin-X cadaverine trifluoroacetic acid salt (5-(((N-(biotinoyl)amino)hexanoyl)amino) pentylamine, trifluoroacetic acid salt) can be coupled to activated carboxylic acid esters or sulfonyl chlorides in the presence of a base or in a basic buffer. Also see biotin-X cadaverine free base (90080).
Biotin-X-C5-maleimide
Biotin-X-C5-maleimide (N-(biotinoyl)-N’-(iodoacetyl)ethylenediamine) is readily and selectively coupled to thiols.
Biotin-X, free acid
Biotin-X free acid (6-((Biotinoyl)amino)hexanoic acid) can be easily converted to the mixed anhydride form, which is more reactive than the succinimidyl ester and thus can be used to react with aromatic amines or sterically hindered amines.
Biotin-XX, free acid
Biotin-XX free acid (6-((6-((Biotinoyl)amino)hexanoyl)amino)hexanoic acid) can be easily converted to the mixed anhydride form, which is more reactive than the succinimidyl ester.
Biotin-X, succinimidyl ester
Biotin-X succinimidyl ester (6-((Biotinoyl)amino)hexanoic acid succinimidyl ester) is readily coupled to a primary or secondary amine under mild conditions.
Biotin-XX, succinimidyl ester
Biotin-XX succinimidyl ester (6-((6-((Biotinoyl)amino)hexanoyl)amino)hexanoic acid succinimidyl ester) is readily coupled to a primary or secondary amine under mild conditions. The longer XX arm facilitates the interaction between biotin and avidin or streptavidin.
Biotin SE
Biotin SE (Biotin, succinimidyl ester) is readily coupled to a primary or secondary amine under mild conditions.
Biotin-rhodamine 110
We developed biotin-rhodamine 110 as an alternative to biotin-4-fluorescein (90062) and fluorescein biotin (80019), both of which have been used for detection of biotin binding sites and the degree of biotinylation of proteins, and for the measurement of avidin and streptavidin in crude biological samples.
Biotin-AM3-25
Biotin-AM3-25 is a conjugate of AM3-25 and biotin. Membranes labeled with the probe can subsequently couple to avidin or streptavidin.
Biotin-11-dCTP, 1 mM Solution
Biotin-11-dCTP (Biotin-11-2′-deoxycytidine-5′-triphosphate, tetralithium salt) can be enzymatically incorporated into DNA via nick translation, random priming, or 3′-end terminal labeling. The number 11 is the number of atoms in the linker between biotin and dCTP.
Biotin-20-UTP, 10 mM Solution
This product is similar to biotin-11-UTP (40033) and biotin-16-UTP (40023) except that it has a longer and water soluble spacer group, which should facilitate interaction between the biotin group and avidin or streptavidin.