Biotinylation Reagents

Biotin-PEO₄-propionate SE (Biotin-PEO₄-propionate succinimidyl ester) is a water soluble biotin derivative that can be readily coupled to primary or secondary amines.

Biotin cadaverine, biotin-X cadaverine (90061), and biotin ethylenediamine (90057) can be coupled to carboxylic acids, DNA and other biomolecules.

Biotin-PEO₃-amine is a water soluble biotin derivative that can be readily coupled to activated carboxylic acids.

Biotin-4-fluorescein (N-(5-aminopentyl)biotinamide, trifluoroacetic acid salt) has been reported to be a superior fluorescent biotin derivative for accurate measurement of avidin and streptavidin in crude biological samples. Binding of biotin to avidin and streptavidin is high affinity, fast and noncooperative.

Biocytin hydrazide is an aldehyde-fixable cellular tracer that can be introduced into cells by microinjection. Biocytin hydrazide can also be used to label glycoproteins or other carbohydrate molecules (See Reactive Dyes for Labeling Glycoproteins and Other Carbohydrate Molecules).

Biotin-X cadaverine trifluoroacetic acid salt (5-(((N-(biotinoyl)amino)hexanoyl)amino) pentylamine, trifluoroacetic acid salt) can be coupled to activated carboxylic acid esters or sulfonyl chlorides in the presence of a base or in a basic buffer. Also see biotin-X cadaverine free base (90080).

Biotin-X-C₅-maleimide (N-(biotinoyl)-N’-(iodoacetyl)ethylenediamine) is readily and selectively coupled to thiols.

Biotin-X free acid (6-((Biotinoyl)amino)hexanoic acid) can be easily converted to the mixed anhydride form, which is more reactive than the succinimidyl ester and thus can be used to react with aromatic amines or sterically hindered amines.

Biotin-XX free acid (6-((6-((Biotinoyl)amino)hexanoyl)amino)hexanoic acid) can be easily converted to the mixed anhydride form, which is more reactive than the succinimidyl ester.

Biotin-X succinimidyl ester (6-((Biotinoyl)amino)hexanoic acid succinimidyl ester) is readily coupled to a primary or secondary amine under mild conditions.

Biotin-XX succinimidyl ester (6-((6-((Biotinoyl)amino)hexanoyl)amino)hexanoic acid succinimidyl ester) is readily coupled to a primary or secondary amine under mild conditions. The longer XX arm facilitates the interaction between biotin and avidin or streptavidin.

Biotin SE (Biotin, succinimidyl ester) is readily coupled to a primary or secondary amine under mild conditions.

We developed biotin-rhodamine 110 as an alternative to biotin-4-fluorescein (90062) and fluorescein biotin (80019), both of which have been used for detection of biotin binding sites and the degree of biotinylation of proteins, and for the measurement of avidin and streptavidin in crude biological samples.

Biotin-AM3-25 is a conjugate of AM3-25 and biotin. Membranes labeled with the probe can subsequently couple to avidin or streptavidin.

Biotin-11-dCTP (Biotin-11-2′-deoxycytidine-5′-triphosphate, tetralithium salt) can be enzymatically incorporated into DNA via nick translation, random priming, or 3′-end terminal labeling. The number 11 is the number of atoms in the linker between biotin and dCTP.

This product is similar to biotin-11-UTP (40033) and biotin-16-UTP (40023) except that it has a longer and water soluble spacer group, which should facilitate interaction between the biotin group and avidin or streptavidin.