Biotinylation Reagents

Biotin-PEO₂-PPO₂-amine trifluoroacetic acid (TFA) salt is a water soluble biotin derivative with an amine group.

Biotin-PEO₃-amine is a water soluble biotin derivative that can be readily coupled to activated carboxylic acids.

Biotin-PEO₄-amine is a water soluble biotin derivative that can be readily coupled to activated carboxylic acids.

Biotin-PEO₄-hydrazide is a water-soluble biotin derivative capable of reacting with aldehydes or ketones to form hydrazones.

Biotin-PEO₄-maleimide is a water soluble biotin derivative that can be readily coupled to thiols.

Biotin-PEO₄-propionate SE (Biotin-PEO₄-propionate succinimidyl ester) is a water soluble biotin derivative that can be readily coupled to primary or secondary amines.

We developed biotin-rhodamine 110 as an alternative to biotin-4-fluorescein (90062) and fluorescein biotin (80019), both of which have been used for detection of biotin binding sites and the degree of biotinylation of proteins, and for the measurement of avidin and streptavidin in crude biological samples.

Biotin-X cadaverine (5-(((N-(Biotinoyl)amino)hexanoyl)amino) pentylamine, free base) can be coupled directly to activated carboxylic acid esters without the use of a base.

Biotin-X cadaverine trifluoroacetic acid salt (5-(((N-(biotinoyl)amino)hexanoyl)amino) pentylamine, trifluoroacetic acid salt) can be coupled to activated carboxylic acid esters or sulfonyl chlorides in the presence of a base or in a basic buffer. Also see biotin-X cadaverine free base (90080).

Biotin-X NTA (Biotin-X nitrilotriacetic acid, tripotassium salt) is used to detect histidine-tagged proteins (as little as 0.1 pmol) immobilized on nitrocellulose membranes.

Biotin-X free acid (6-((Biotinoyl)amino)hexanoic acid) can be easily converted to the mixed anhydride form, which is more reactive than the succinimidyl ester and thus can be used to react with aromatic amines or sterically hindered amines.

Biotin-X succinimidyl ester (6-((Biotinoyl)amino)hexanoic acid succinimidyl ester) is readily coupled to a primary or secondary amine under mild conditions.

Biotin-X-C₅-maleimide (N-(biotinoyl)-N’-(iodoacetyl)ethylenediamine) is readily and selectively coupled to thiols.

Biotin-X-DHPE (N-((6-(Biotinoyl)amino)hexanoyl)-1,2-dihexadecanoylsn-glycero-3-phosphoethanolamine, triethylammonium salt) can be used to couple avidin or streptavidin to cell membranes or liposomes.

Biotin-XX free acid (6-((6-((Biotinoyl)amino)hexanoyl)amino)hexanoic acid) can be easily converted to the mixed anhydride form, which is more reactive than the succinimidyl ester.

Biotin-XX succinimidyl ester (6-((6-((Biotinoyl)amino)hexanoyl)amino)hexanoic acid succinimidyl ester) is readily coupled to a primary or secondary amine under mild conditions. The longer XX arm facilitates the interaction between biotin and avidin or streptavidin.