Reactive CF® Dyes, Other Reactive Dyes & Biotinylation Reagents

5(6)-ROX [full name: 5-(and-6)-Carboxy-X-rhodamine, triethylammonium salt (mixed isomers)] is a long wavelength rhodamine dye.

5-(and-6)-Carboxyrhodamine 110, SE (5-(and-6)-Carboxyrhodamine 110, succinimidyl ester, mixed isomers), also known as Carboxyrhodamine 110-NHS, is a superior green fluorescent dye that readily reacts with primary or secondary amines.

5-(and-6)-Carboxyrhodamine 110-X (full name: 5-(and-6)-Carboxyrhodamine 110-X, hydrochloride) is similar to 5-(and-6)-carboxyrhodamine 110 (#90000).

The thiol-reactive quencher, CFQ520 maleimide, has a broad and intense absorption (approximately 520 nm) but no fluorescence, making it useful as an acceptor in fluorescence resonance energy transfer (FRET) applications.

For some applications, 5-(and-6)-carboxyrhodamine (5(6)-CR 110) is a superior alternative to the widely used green fluorescent dye 5-(and-6)-carboxyfluorescein. The dye is insensitive to pH between 4 and 9 and is much more photostable than fluorescein.

NFQ1, a non-fluorescent quencher, is used for a new type of One Sample Fluorescence Resonance Energy Transfer (OS-FRET) method employing a novel, non-fluorescent methanethiosulfonate-linked acceptor that can be reversibly coupled to a target sulfhydryl residue via a disulfide bond.

The amine-reactive quencher, CFQ520 succinimidyl ester, has a broad and intense absorption (approximately 520 nm) but no fluorescence, making it useful as an acceptor in fluorescence resonance energy transfer (FRET) applications.

We developed biotin-rhodamine 110 as an alternative to biotin-4-fluorescein (90062) and fluorescein biotin (80019), both of which have been used for detection of biotin binding sites and the degree of biotinylation of proteins, and for the measurement of avidin and streptavidin in crude biological samples.

Like Lucifer Yellow CH, Lucifer Yellow Cadaverine can be used as a fixable fluorescent tracer. In addition, the dye can be used to conjugate to carboxylic acid groups.

Biotin-AM3-25 is a conjugate of AM3-25 and biotin. Membranes labeled with the probe can subsequently couple to avidin or streptavidin.

6-FAM (full name: 6-Carboxyfluorescein, single isomer) is the free acid form of 6-carboxyfluorescein single isomer.

5-FAM (full name: 5-Carboxyfluorescein, single isomer) is the free acid form of 5-carboxyfluorescein single isomer.

Biotin-11-dCTP (Biotin-11-2′-deoxycytidine-5′-triphosphate, tetralithium salt) can be enzymatically incorporated into DNA via nick translation, random priming, or 3′-end terminal labeling. The number 11 is the number of atoms in the linker between biotin and dCTP.

This product is similar to biotin-11-UTP (40033) and biotin-16-UTP (40023) except that it has a longer and water soluble spacer group, which should facilitate interaction between the biotin group and avidin or streptavidin.

Biotin-11-CTP (Biotin-11-cytidine-5′-triphosphate, tetralithium salt) can be enzymatically incorporated into RNA.

Biotin-11-UTP can be enzymatically incorporated into RNA. The number 11 is the number of atoms in the linker between biotin and UTP.