Reactive CF® Dyes, Other Reactive Dyes & Biotinylation Reagents

Biotin-PEO₂-PPO₂-amine trifluoroacetic acid (TFA) salt is a water soluble biotin derivative with an amine group.

Biotin cadaverine free base (N-(5-Aminopentyl)biotinamide) can be directly coupled to activated carboxylic acid groups without using an additional base.

Biotin ethylenediamine, hydrochloride is functionally equivalent to Neurobiotin™ (biotin ethylenediamine, hydrobromide, 90057).

AMCA-X SE is an amine-reactive, UV-excitable, blue fluorescent dye.

ARP (N-(Aminooxyacetyl)-N’-(D-biotinoyl)hydrazine, trifluoroacetic acid salt) reacts with the exposed aldehyde group formed at abasic sites in damaged DNA, allowing the DNA to be labeled with biotin groups (1,2). The labeled DNA can then be quantitated with fluorescent or enzyme-conjugated streptavidin complexes.

Biotin-PEO₄-amine is a water soluble biotin derivative that can be readily coupled to activated carboxylic acids.

Biotin-PEO₄-propionate SE (Biotin-PEO₄-propionate succinimidyl ester) is a water soluble biotin derivative that can be readily coupled to primary or secondary amines.

Biotin cadaverine, biotin-X cadaverine (90061), and biotin ethylenediamine (90057) can be coupled to carboxylic acids, DNA and other biomolecules.

Biotin-PEO₃-amine is a water soluble biotin derivative that can be readily coupled to activated carboxylic acids.

Biotin-4-fluorescein (N-(5-aminopentyl)biotinamide, trifluoroacetic acid salt) has been reported to be a superior fluorescent biotin derivative for accurate measurement of avidin and streptavidin in crude biological samples. Binding of biotin to avidin and streptavidin is high affinity, fast and noncooperative.

Biocytin hydrazide is an aldehyde-fixable cellular tracer that can be introduced into cells by microinjection. Biocytin hydrazide can also be used to label glycoproteins or other carbohydrate molecules (See Reactive Dyes for Labeling Glycoproteins and Other Carbohydrate Molecules).

Biotin-X cadaverine trifluoroacetic acid salt (5-(((N-(biotinoyl)amino)hexanoyl)amino) pentylamine, trifluoroacetic acid salt) can be coupled to activated carboxylic acid esters or sulfonyl chlorides in the presence of a base or in a basic buffer. Also see biotin-X cadaverine free base (90080).

Biotin-X-C₅-maleimide (N-(biotinoyl)-N’-(iodoacetyl)ethylenediamine) is readily and selectively coupled to thiols.

Biotin-X free acid (6-((Biotinoyl)amino)hexanoic acid) can be easily converted to the mixed anhydride form, which is more reactive than the succinimidyl ester and thus can be used to react with aromatic amines or sterically hindered amines.

Biotin-XX free acid (6-((6-((Biotinoyl)amino)hexanoyl)amino)hexanoic acid) can be easily converted to the mixed anhydride form, which is more reactive than the succinimidyl ester.

Biotin-X succinimidyl ester (6-((Biotinoyl)amino)hexanoic acid succinimidyl ester) is readily coupled to a primary or secondary amine under mild conditions.