Tech Tip: Fluorescent Detection of EVs by Flow Cytometry

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Extracellular vesicles (EVs) are small, membrane-bound particles secreted from cells and thought to function as cellular messengers, carrying cargo from one cell to another. In biomedical research, EVs and their cargo are used as diagnostic biomarkers for cancer and other diseases. However, the isolation and detection of EVs can be extremely challenging due to their small size (~30-200 nm in diameter, similar to most viruses). New methods and tools are constantly being developed but it can be difficult to know which to use.

In this tech tip, Biotium scientists share their expertise for optimal fluorescent staining and detection of EVs by flow cytometry. To learn about EVs and EV biology, see our recent blog post on the subject.

The article includes:

• A detailed comparison of EV isolation methods
• Tips for using fluorescent antibodies or membrane dyes to label EVs
• Best practices for flow cytometry detection of EVs