Biotium Launches First Ever Mounting Media with Far-Red Nuclear Counterstain and Autofluorescence Quenching Reagent

Fremont, CA, June 29, 2021: Biotium, a leading provider of reagents for immunofluorescence microscopy, is significantly expanding its line of antifade wet-set and hardset EverBrite™ Mounting Medium with options that include our new far-red NucSpot® 640 nuclear counterstain. In addition, Biotium is also releasing EverBrite TrueBlack® Hardset Mounting Medium for mounting and quenching of lipofuscin autofluorescence in a single step.

Antifade mounting media, like EverBrite™, typically includes DAPI as a convenient blue nuclear counterstain for researchers performing immunofluorescence microscopy. However, cross-talk between fluorescence channels and UV-induced photoconversion of DAPI can cause bleed-through fluorescence in neighboring FITC or Cy®3 channels (see our related tech tip). Biotium developed EverBrite™ Mounting Media with NucSpot® counterstains as a novel solution to DAPI cross-talk and UV photoconversion, offering bright and specific nuclear imaging with very limited interference in other channels. EverBrite™ NucSpot® 640 is specific for the Cy®5 channel.

“Mounting medium has traditionally only provided antifade properties and a convenient DAPI counterstain, but does little to address other issues with fluorescence.” says Lori Roberts PhD, Director of Bioscience at Biotium. “Our new EverBrite™ products aim to provide both convenience and novel research solutions for our customers.”