Biotium Expands Cheetah™ Taq Portfolio with New Formulations for Fast Cycling PCR and Proximity Extension Assays

Cheetah™ FLEX HotStart Taq: Fast-Cycling & PEA Versatility

Cheetah™ FLEX is engineered for speed and adaptability across a range of applications. With a rapid 2-minute activation at 95°C, FLEX delivers up to 10x faster activation than AmpliTaq Gold® or HotStartTaq®, with none of the drawbacks of antibody-based hot-start enzymes. The antibody-free formulation eliminates animal DNA contamination and provides enhanced long-term stability. Cheetah™ FLEX is validated for fast-cycling PCR and qPCR-based PEA, making it a go-to choice for both routine and advanced workflows. After heat activation, Cheetah™ Taq has the same functional properties and fidelity as native Taq polymerase.

Cheetah™ PEA-PLEX HotStart Taq: Optimized for NGS-Based High-Throughput PEA

Cheetah™ PEA-PLEX is a unique formulation of Cheetah™ Taq developed  specifically for high-throughput, NGS-based PEA assay for protein detection. Third-party validated in high-throughput PEA, Cheetah™ PEA-PLEX is also compatible with fast-cycling qPCR.

Key Features of Cheetah™ HotStart Taq DNA Polymerase:

• Novel chemical hot-start mechanism: No antibodies, no activation lag
• Rapid 2-minute activation at 95°C
• Antibody-free, low-DNA enzyme for contamination-free results
• Superior long-term stability than traditional chemically modified Taq
• Versatile use for fast-cycling qPCR and PEA
• Cheetah™ PEA-PLEX developed and validated for NGS-based PEA assays
• Available in ready-to-use kits and customizable bulk formats

Cheetah™ FLEX and Cheetah™ PEA-PLEX Hotstart Taq are available now and can be purchased in standard or bulk sizes. Both formulations are available for licensing; contact techsupport@biotium.com for evaluation samples and to discuss custom size, packaging, or formulation options.

Learn more about Cheetah™ FLEX HotStart Taq DNA Polymerase and Cheetah™ PEA-PLEX HotStart Taq DNA Polymerase. These technologies add to Biotium’s growing portfolio of high-performance PCR reagents, including Forget-Me-Not™ qPCR Master Mixes, powered by Cheetah™ Taq and EvaGreen® qPCR Dye.