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Cheetah™ HotStart Taq DNA Polymerase

A hot-start Taq DNA polymerase modified using a novel modifying reagent, it has a faster activation time and better shelf-life.

Product Attributes



Storage Conditions

Store at -10 to -35 °C

Catalog #
500 U
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Product Description

Cheetah™ Taq is a chemically modified hot-start Taq DNA polymerase useful for preventing or reducing nonspecific DNA amplification in PCR. Modified using a novel modifying reagent, Cheetah™ Taq represents a major improvement upon AmpliTaq® Gold by having a faster activation time and better shelf-life.


  • Hot-start Taq polymerase
  • Modified using unique hot-start chemistry
  • Absolutely no activity at room temperature
  • Fast activation: takes less than 2 minutes of heating to activate
  • Very stable during storage


  • Cheetah™ Taq, 5 U/uL, 100 uL
    (in buffer containing Tris-HCl pH 9.0, DTT, EDTA, KCl and glycerol)
  • 10X Cheetah™ Taq Dilution Buffer (without Mg2+), 1.5 mL
  • 25 mM MgCl2, 1.5 mL

Under standard hot-start conditions (i.e., 94°C in pH 8-9 Tris), Cheetah™ Taq regains both the 3′-5′ polymerase and 5′-exonuclease activities within two minutes. Cheetah™ Taq is also superior to antibody-based hot-start DNA polymerases due to its complete suppression of enzyme activity at room temperature and full recovery of enzyme activity following activation.

AmpliTaq is a registered trademark of Thermo Fisher Scientific.

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