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Eosin-5-isothiocyanate

Eosin-5-isothiocyanate is a good photosensitizer and can be used to selectively label amines. It has a quantum yield of 0.

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Product Description

Eosin-5-isothiocyanate is a good photosensitizer and can be used to selectively label amines. It has a quantum yield of 0.57 for singlet oxygen generation, which makes it useful for photoconversion of electron-rich materials such as diaminobenzidine (DAB) or fluorescent dyes into highly electron-dense materials for high resolution electron microscopy studies (1,2). Additionally, eosin is a good fluorescence acceptor for fluorescein (FAM), dansyl and coumarin dyes in fluorescence resonance energy transfer (FRET).

  • λExEm (pH9) = 521/544 nm
  • ε = 95,000
  • Orange red solid soluble in DMF or DMSO
  • Store at -20°C and protect from light
  • C21H7Br4NO5S
  • MW: 704.96

References

1. J Cell Biol 126, 901 (1994).

2. J Cell Biol 126, 877 (1994).

 

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