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NBD Chloride

NBD chloride (4-Chloro-7-nitrobenzo-2-oxa-1,3-diazole) is nonfluorescent until it reacts with primary or secondary amines to produce a fluorescent product. NBD chloride has been extensively used as a derivatizing reagent for chromatography analysis of amino acids and low molecular weight amines.

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Product Description

NBD chloride (4-Chloro-7-nitrobenzo-2-oxa-1,3-diazole) is nonfluorescent until it reacts with primary or secondary amines to produce a fluorescent product. NBD chloride has been extensively used as a derivatizing reagent for chromatography analysis of amino acids and low molecular weight amines.

  • λExEm(MeOH) = 465/535 nm (for reaction product with primary amines); 485/540 nm (for reaction product with secondary amines)
  • Yellow solid soluble in DMF
  • Store at -20°C and protect from light
  • C6H2ClN3O3
  • MW: 199.55

References

1. Biochem J 108, 155 (1968).

2. Anal Biochem 116, 471 (1981).

3. Anal Chim Acta 290, 3 (1994).

 

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