NBD Chloride
NBD chloride (4-Chloro-7-nitrobenzo-2-oxa-1,3-diazole) is nonfluorescent until it reacts with primary or secondary amines to produce a fluorescent product. NBD chloride has been extensively used as a derivatizing reagent for chromatography analysis of amino acids and low molecular weight amines.
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Product Description
NBD chloride (4-Chloro-7-nitrobenzo-2-oxa-1,3-diazole) is nonfluorescent until it reacts with primary or secondary amines to produce a fluorescent product. NBD chloride has been extensively used as a derivatizing reagent for chromatography analysis of amino acids and low molecular weight amines.
- λEx/λ Em(MeOH) = 465/535 nm (for reaction product with primary amines); 485/540 nm (for reaction product with secondary amines)
- Yellow solid soluble in DMF
- Store at -20°C and protect from light
- C6H2ClN3O3
- MW: 199.55
References
1. Biochem J 108, 155 (1968).
2. Anal Biochem 116, 471 (1981).
3. Anal Chim Acta 290, 3 (1994).