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NBD Fluoride

NBD fluoride (4-Fluoro-7-nitrobenzo-2-oxa-1,3-diazole) is similar to NBD chloride (90045) except that the former is much more reactive toward amines.

Product Attributes


465/535 nm (reaction product with primary amine); 485/540 nm (reaction product with secondary amine)

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25 mg
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Product Description

NBD fluoride (4-Fluoro-7-nitrobenzo-2-oxa-1,3-diazole) is similar to NBD chloride (90045) except that the former is much more reactive toward amines. Both reagents are nonfluorescent until they react with primary or secondary amines to produce a fluorescent product. NBD chloride and NBD fluoride have been extensively used as derivatizing reagents for chromatography analysis of amino acids and low molecular weight amines.

  • λExEm (MeOH) = 465/535 nm (for reaction product with primary amines); 485/540 nm (for reaction product with secondary amines)
  • Yellow solid soluble in DMF
  • Store at -20°C and protect from light
  • C6H2FN3O3
  • MW:183


1. Anal Chim Acta 130, 377 (1981).

2. Biochem J 108, 155 (1968).

3. Anal Biochem 116, 471 (1981).

4. Anal Chim Acta 290, 3 (1994).


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    NBD-PE (N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)-1,2-dihexadecanoyl-snglycero-3-phosphoethanolamine, triethylammonium salt) in combination with Rhodamine-DHPE (#60026) or Texas Red®-DHPE (#60027) has been used to study membrane fusion via fluorescence resonance energy transfer (FRET) (1-3). NBD-PE has also been used in photobleaching recovery measurement.

    NBD C6-Sphingomyelin

    NBD C6-sphingomyelin (6-((N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)hexanoyl)sphingosyl phosphocholine) has been used for following sphingolipid metabolism in cells.

    NBD C6-Ceramide

    NBD C6-ceramide (6-((N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)hexanoyl)sphingosine) has been used for following sphingolipid metabolism in cells, and as a selective stain for the Golgi apparatus in live and fixed cells.

    DMSO, Anhydrous

    Anhydrous DMSO (dimethyl sulfoxide; methyl sulfoxide) is recommended for preparing stock solutions of AM ester dyes, reactive dyes and other related moisture-sensitive products.

    FAM SE, 5 and 6 isomers

    5(6)-FAM SE (full name: 5-(and-6)-Carboxyfluorescein, succinimidyl ester mixed isomers) is an amine-reactive green fluorescent dye widely used for labeling proteins or other molecules that contain a primary or secondary aliphatic amine. The coupling reaction is usually carried out at pH 8-9.5.