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RNaseReveal™ Activity Assay Kit

Fluorescent assay for sensitive detection of RNase activity in liquid samples. Ideal for detecting RNase contamination in quality control workflows.

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Product Description

The RNaseReveal™ Activity Assay Kit is a fluorescent assay for the detection of RNase activity in liquid samples. The buffer and substrate have been formulated for maximum sensitivity, making it a useful tool for detecting RNase contamination in quality control workflows. Biotium also offers RNase A (Cat. No. 99871-600uL) as a separate product that is recommended for use with this kit.

Features

  • Exceptional sensitivity, as little as ~0.08 pg RNase
  • Simple 40 minute assay
  • Detect with handheld fluorometer or fluorescence plate reader
  • Useful for detecting RNase contamination in QC workflows

Kit components

  • RNaseReveal Substrate™ (Cat. No. 31086A)
  • 10X RNaseReveal™ Assay Buffer (Cat. No. 31086B)

The kit utilizes RNaseReveal™ Substrate which is an RNA probe tagged with a green fluorophore and a quencher, so that the intact probe is non-fluorescent. In the presence of RNase, the probe is cleaved and the fluorophore is detached from the quencher, releasing a green fluorescent signal. The lower limit of detection of RNase in the assay is ~ 0.08 pg, using Biotium’s RNase A (Cat. No. 99871-600uL) at ≥50 U/mg (Kunitz units). Full substrate cleavage is achieved with ~ 800 pg of the same RNase A. The excitation and emission maximum of the substrate is 496/516 nm. The substrate can be detected by any fluorescence plate reader or fluorometer capable of detecting green fluorescence. Biotium also offers RNase A (Cat. No. 99871-600uL) for use with this kit.

A dilution series of RNase A (Cat. No. 99871-600uL) was prepared and 800 pg down to 0.08 pg was tested in the RNaseReveal™ assay. The lowest amount of RNase A (0.08 pg) was detectable above background. The signal saturated at 800 pg/well.

Learn more about Biotium’s unique products for RNA workflows, including RNA extraction kits, quantitation kits, and RNA stains for gel electrophoresis.

 

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