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RNAstorm™ Kit for Isolation of RNA from FFPE Tissue Samples

Superior RNA extraction from FFPE samples. 3 to 30-fold increase in yield. 2-fold increase in DV200 (RNA over 200 nuc).

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Product Description

Formalin-fixed tissue samples are a challenge for RNA extraction, often resulting in low yields of amplifiable RNA and poor performance in subsequent steps involving enzymatic manipulation, including reverse transcription and sequencing library preparation.

Powered by proprietary CAT5™ technology, the RNAstorm™ extraction kit enhances the removal of formaldehyde-induced damage and provides RNA with higher yield and quality, better integrity, and greater amplifiability.

Whether you are performing RNA-seq, qPCR, microarray or other gene expression analysis, the RNAstorm™ kit is your best chance for success.

We also offer the DNAstorm™ kit for extraction of high-quality DNA from FFPE tissue samples.

The RNAstorm™ and DNAstorm™ kits can be combined to obtain both pure DNA and RNA from the same tissue section. See our App Note under Downloads.

Advantages of the RNAstorm™ Kit

Higher Yields of Amplifiable RNA

Significant improvements in RNA yield and quality (as measured by amount of amplifiable RNA) are seen using a prototype of the RNAstorm™ kit on FFPE samples from various tissues and ranging in age from 1976 to 2015.

 

Comparison of RNA recovery by quantitative RT-PCR from FFPE tissues. “Q” represents a competitive commercial FFPE extraction kit.

 

Higher Integrity RNA

Increased DV200 values are observed for RNA extracted using the RNAstorm™ kit relative to a popular commercial kit. The DV200 represents percentage of RNA with length greater than 200 nt, as measured using an Agilent Bioanalyzer RNA 6000 nano kit.

 

An exemplary overlay of BioAnalyzer traces obtained using RNA from the same sample as seen with the RNAstorm™ kit and Kit Q is shown below.
 

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