Biotin & Streptavidin Conjugates

We developed biotin-rhodamine 110 as an alternative to biotin-4-fluorescein (90062) and fluorescein biotin (80019), both of which have been used for detection of biotin binding sites and the degree of biotinylation of proteins, and for the measurement of avidin and streptavidin in crude biological samples.

Biotin-X cadaverine (5-(((N-(Biotinoyl)amino)hexanoyl)amino) pentylamine, free base) can be coupled directly to activated carboxylic acid esters without the use of a base.

Biotin-X cadaverine trifluoroacetic acid salt (5-(((N-(biotinoyl)amino)hexanoyl)amino) pentylamine, trifluoroacetic acid salt) can be coupled to activated carboxylic acid esters or sulfonyl chlorides in the presence of a base or in a basic buffer. Also see biotin-X cadaverine free base (90080).

Biotin-X NTA (Biotin-X nitrilotriacetic acid, tripotassium salt) is used to detect histidine-tagged proteins (as little as 0.1 pmol) immobilized on nitrocellulose membranes.

Biotin-X free acid (6-((Biotinoyl)amino)hexanoic acid) can be easily converted to the mixed anhydride form, which is more reactive than the succinimidyl ester and thus can be used to react with aromatic amines or sterically hindered amines.

Biotin-X succinimidyl ester (6-((Biotinoyl)amino)hexanoic acid succinimidyl ester) is readily coupled to a primary or secondary amine under mild conditions.

Biotin-X-C₅-maleimide (N-(biotinoyl)-N’-(iodoacetyl)ethylenediamine) is readily and selectively coupled to thiols.

Biotin-X-DHPE (N-((6-(Biotinoyl)amino)hexanoyl)-1,2-dihexadecanoylsn-glycero-3-phosphoethanolamine, triethylammonium salt) can be used to couple avidin or streptavidin to cell membranes or liposomes.

Biotin-XX tyramide conjugate can be used for tyramide signal amplification (TSA) to generate high-density labeling of a target protein or nucleic acid with biotin.

Biotin-XX free acid (6-((6-((Biotinoyl)amino)hexanoyl)amino)hexanoic acid) can be easily converted to the mixed anhydride form, which is more reactive than the succinimidyl ester.

Biotin-XX succinimidyl ester (6-((6-((Biotinoyl)amino)hexanoyl)amino)hexanoic acid succinimidyl ester) is readily coupled to a primary or secondary amine under mild conditions. The longer XX arm facilitates the interaction between biotin and avidin or streptavidin.

A high affinity inhibitor of the nicotinic acetylcholine receptor in the neuromuscular junction. Blocks acetylcholine activity at the postsynaptic membrane. The biotin conjugate can be subsequently labeled with anti-biotin antibodies, avidin, or streptavidin for signal amplification.

Fluorescein biotin (5-((N-(5-(N-(6-(Biotinoyl)amino)hexanoyl)amino)pentyl)thioureidyl) fluorescein) can be used to detect and quantify biotin binding sites and the degree of biotinylation of proteins. Also see biotin-rhodamine 110 (80022) and biotin-4-fluorescein (90062).

This fluorescent biotin derivative is a useful tracer for studying neuronal morphology. The biotin portion of the molecule allows researchers to amplify the fluorescent signal using standard biotin-avidin or biotin-streptavidin biochemistry.

Label your antibody with biotin in just 15 minutes without a purification step. Labeling tolerates many common buffer components including BSA and ascites.