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Biotin-XX-α-Bungarotoxin

A high affinity inhibitor of the nicotinic acetylcholine receptor in the neuromuscular junction. Blocks acetylcholine activity at the postsynaptic membrane. The biotin conjugate can be subsequently labeled with anti-biotin antibodies, avidin, or streptavidin for signal amplification.

Product Attributes

Toxin

Alpha-bungarotoxin

For live or fixed cells

For fixed cells, For live/intact cells

Cell permeability

Membrane impermeant

Fixation options

Permeabilize before staining, Fix before staining (methanol), Fix before staining (formaldehyde)

Conjugation

Biotin

Storage Conditions

Store at -10 to -35 °C, Protect from light

Size
Catalog #
price
Qty
0.5 mg
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Product Description

A potent neurotoxin that is an inhibitor for the motor endplate acetylcholine receptor (Kd = 1 nM to 1 pM). It binds at the neuromuscular junction with high affinity and is often used to study neuromuscular junctions by blocking cholinergic receptors. The toxin also blocks α7 receptors, located in the central and peripheral nervous systems. The biotin conjugate can be subsequently labeled with anti-biotin antibodies, avidin, or streptavidin for signal amplification.

  • A polypeptide snake toxin
  • Blocks and inhibits cholinergic receptors
  • Biotinylated for avidin or streptavidin reactivity and signal amplification
  • White solid soluble in water

Biotinylated α-bungarotoxin is useful in the affinity column isolation of the nicotinic AChR using an avidin or streptavidin agarose (1). Nicotinic AChR labeled with biotin-XX-α-bungarotoxin can be localized using enzyme or fluorophore-labeled conjugates of avidin or streptavidin (2,3).

We also offer unlabeled α-bungarotoxin and CF® Dye α-Bungarotoxin conjugated to our next-generation fluorescent dyes. See our complete selection of α-Bungarotoxin Products below.

α-Bungarotoxin & Conjugates

ProductEx/Em SizeCatalog number
α-BungarotoxinN/A1 mg00010-1
Biotin-XX-α-BungarotoxinN/A0.5 mg00017
α-Bungarotoxin, CF®405S Conjugate404/431 nm100 ug00002-100ug
0.5 mg00002
α-Bungarotoxin, CF®488A Conjugate490/515 nm100 ug00005-100ug
0.5 mg00005
α-Bungarotoxin, CF®543 Conjugate541/560 nm100 ug00026-100ug
0.5 mg00026
α-Bungarotoxin, CF®555 Conjugate555/565 nm100 ug00018-100ug
0.5 mg00018
α-Bungarotoxin, CF®568 Conjugate562/583 nm100 ug00006-100ug
0.5 mg00006
α-Bungarotoxin, CF®594 Conjugate596/614 nm100 ug00007-100ug
0.5 mg00007
α-Bungarotoxin, CF®633 Conjugate630/650 nm100 ug00009-100ug
0.5 mg00009
α-Bungarotoxin CF®640R, Conjugate642/662 nm100 ug00004-100ug
0.5 mg00004
α-Bungarotoxin CF®680R, Conjugate680/701 nm100 ug00003-100ug
0.5 mg00003
α-Bungarotoxin, Fluorescein (FITC) Conjugate494/518 nm0.5 mg00011
10 x 50 ug00013
α-Bungarotoxin, Tetramethylrhodamine Conjugate553/577 nm0.5 mg00012
10 x 50 ug00014
α-Bungarotoxin, Sulforhodamine-101 (Texas Red®) Conjugate593/613 nm0.5 mg00015
10 x 50 ug00016
CF is a registered trademark of Biotium, Inc. Texas Red is a registered trademark of Thermo Fisher Scientific.

References

  1. PLoS One, 8, 7, (2013), DOI: 10.1371/journal.pone.0070338
  2. Am J of Pathol, 177, 2509 (2010), DOI: 10.2353/ajpath.2010.100243
  3. Cell Tissue Biol, 4, 258, (2010), DOI: 10.1134/S1990519X10030077
  4. Neuroscience, 174, 234, (2009), DOI: 10.1016/j.neuroscience.2010.11.016
  5. J Cell Biol, 150, 1385, (2000), DOI: 10.1083/jcb.150.6.1385
  6. Neuron, 23, 675, (1999), DOI: 10.1016/s0896-6273(01)80027-1
  7. Neuron, 12, 167, (1994), DOI: 10.1016/0896-6273(94)90161-9
  8. J Cell Biol, 125, 661, (1994), DOI: 10.1083/jcb.125.3.661
  9. J Biol Chem, 268, 25108, (1993), PMID: 8227074
  10. Muscle Nerve, 5, 140, (1982), DOI: 10.1002/mus.880050211
  11. PNAS 77, 4823, (1980), DOI: 10.1073/pnas.77.8.4823
  12. Science, 196, 540, (1977), DOI: 10.1126/science.850796
 

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