PCR & DNA Amplification

Highly purified dNTPs (>99% by HPLC) in purified water. Each deoxynucleotide solution (dATP, dCTP, dGTP, dTTP) is provided in a separate vial at 100 mM.

A simple kit for reversibly modifying non-thermostable enzymes like reverse transcriptase, rendering the enzyme inactive during reaction set-up. Activity is restored by heating to 45°C or higher.

A patented green fluorescent nucleic acid dye with features that are ideal for applications such as qPCR, HRM®, LAMP, and digital PCR. Highly concentrated for customers with specialized applications requiring a concentrated dye solution.

ARP (N-(Aminooxyacetyl)-N’-(D-biotinoyl)hydrazine, trifluoroacetic acid salt) reacts with the exposed aldehyde group formed at abasic sites in damaged DNA, allowing the DNA to be labeled with biotin groups (1,2). The labeled DNA can then be quantitated with fluorescent or enzyme-conjugated streptavidin complexes.

A 5X concentrated buffer solution in deionized water. TBE is used for polyacrylamide and agarose gel electrophoresis.

A patented green fluorescent nucleic acid dye with features that are ideal for applications such as qPCR, HRM®, LAMP, and digital PCR.

A qPCR master mix containing Evagreen® qPCR dye and Cheetah™ Taq hotstart DNA polymerase, suitable for qPCR using a fast cycling protocol.

A dye used to normalize well-to-well variations that may occur during quantitative, real-time PCR reactions.

A simple kit for reversibly modifying thermostable DNA polymerases, rendering the enzyme inactive and preventing primer dimers during reaction set-up.

An easy and accurate way to determine the activity of a DNA polymerase without using radioisotopes.

This item is discontinued.

Activated charcoal in a convenient bag for decontamination of used gel staining solutions containing ethidium bromide or other toxic dyes.

DODC (DiOC₂(5)) has been reported to bind to triplex DNA as well as dimeric hairpin quadruplex DNA (1). Binding of the dye to triplex DNA quenches the fluorescence of the dye and red-shifts the dye absorption peak.

DiSC₂(5) (3,3′-Diethylthiadicarbocyanine, iodide) binds to DNA-DNA or PNA-DNA duplexes with a large blue-shift of the absorption spectrum. Binding of the dye to PNA-DNA hybrid, in particular, blue-shifts the dye absorption spectrum by as much as 115 nm, offering a visual method to detect nucleic acid hybridization (1,2).

PAGE GelRed® is a non-toxic, non-mutagenic red DNA gel stain specifically formulated to stain DNA in polyacrylamide gels. PAGE GelRed® can be imaged using a 254 nm UV transilluminator with an ethidium bromide filter.

A high-performance product for fluorescent probe-based PCR applications, including quantitation and SNP genotyping.