Reactive CF® Dyes, Other Reactive Dyes & Biotinylation Reagents

Aminooxy-5(6)-TAMRA reacts with aldehydes or ketones to form a stable oxime linkage under mild conditions. It can also be used to label abasic sites in damaged DNA.

Aminooxy-biotin can be used to covalently attach biotin to aldehyde or ketone groups on polysaccharides, glycoproteins or antibodies. Aminooxy-biotin has also been used to efficiently label cell-surface glycans on living animal cells.

Aniline, 10X in acetate buffer is designed to use as catalytic reagent for the optimal coupling of protein carbonyls with aminooxy compounds.

ANTS (8-aminonaphthalene-1,3,6-trisulfonic acid, disodium salt) is a highly negatively charged dye with an amino group that can be coupled to an aldehyde or ketone group to form an unstable Schiff base. The Schiff base is usually chemically reduced by sodium borohydride (NaBH₄) or sodium cyanoborohydride (NaB(CN)H₃) to form a stable linkage (1).

APTS is a very useful green fluorescent dye for labeling glycoproteins or sugar molecules in general. The labeling occurs via reductive amination which involves the formation of a Schiff base between the amine of APTS and the aldehyde or ketone of the sugar, followed by the reduction of the Schiff base linkage to a stable carbon-nitrogen bond.

ARP (N-(Aminooxyacetyl)-N’-(D-biotinoyl)hydrazine, trifluoroacetic acid salt) reacts with the exposed aldehyde group formed at abasic sites in damaged DNA, allowing the DNA to be labeled with biotin groups (1,2). The labeled DNA can then be quantitated with fluorescent or enzyme-conjugated streptavidin complexes.

Biocytin hydrazide is an aldehyde-fixable cellular tracer that can be introduced into cells by microinjection. Biocytin hydrazide can also be used to label glycoproteins or other carbohydrate molecules (See Reactive Dyes for Labeling Glycoproteins and Other Carbohydrate Molecules).

Biotin alkyne reacts with azide to form 1,2,3-triazole by copper(I) catalyzed 1,3-dipolar Huisgen cycloaddition.

Biotin azide reacts with alkyne to form 1,2,3-triazole by copper(I) catalyzed 1,3-dipolar Huisgen cycloaddition.

Biotin BCN, an alternative to biotin labeled DIBO and DBCO, reacts with azide to form 1,2,3-triazole by copper free 1,3-dipolar Huisgen cycloaddition.

Biotin cadaverine free base (N-(5-Aminopentyl)biotinamide) can be directly coupled to activated carboxylic acid groups without using an additional base.

Biotin cadaverine, biotin-X cadaverine (90061), and biotin ethylenediamine (90057) can be coupled to carboxylic acids, DNA and other biomolecules.

Biotin ethylenediamine, hydrochloride is functionally equivalent to Neurobiotin™ (biotin ethylenediamine, hydrobromide, 90057).

MTSEA biotin selectively labels thiols under mild conditions. It’s available in 3 variations: Biotin, Biotin-X, and Biotin-XX, all of which allow selective labeling of cell surface thiols.

Biotin NTA, also known as BNTA (Biotin nitrilotriacetic acid, tripotassium salt) is used to detect histidine-tagged proteins (as little as 0.1 pmol) immobilized on nitrocellulose membranes.