Reactive CF® Dyes, Other Reactive Dyes & Biotinylation Reagents

5-CR110-PEO₄ SE (full name: 5-Carboxyrhodamine 110-PEO₄-propionate, succinimidyl ester, single isomer) is an excellent green fluorescent rhodamine dye for labeling amines. The dye has better solubility than the parent dye 5-CR110 SE and its long PEG spacer should reduce any steric effect of the dye on the molecule to be labeled.

Amine-reactive form of single isomer carboxyrhodamine 110. For certain applications, the dye is preferred over the corresponding 6-carboxyfluorescein because of its exceptional photostability and fluorescence insensitivity to pH change (4-9).

Amine-reactive form of single isomer carboxyrhodamine 110, useful in DNA sequencing. For many single-dye-labeling applications, the dye is preferred over other green fluorescent dyes including fluorescein and Alexa Fluor® 488 because of its exceptional photostability and fluorescence insensitivity to pH.

5-CR110-X, SE (full name: 5-Carboxyrhodamine 110-X, succinimidyl ester) is the amine-reactive form of 5-carboxyrhodamine 110 with a 6-carbon spacer.

N-(1,10-Phenanthrolin-5-yl)iodoacetamide has been used to label thiols to confer the metal-binding properties of the phenanthroline chelator. For example, completing of the chelator with copper can be used to selectively cleave RNA or DNA in the presence of hydrogen peroxide.

Tetramethylrhodamine (TAMRA) cadaverine (full name: 5-(and-6-)-((N-(5-Aminopentyl)amino)carbonyl)tetramethylrhodamine) can be coupled to activated carboxy groups or sulfonyl chlorides.

5(6)-Carboxytetramethylrhodamine maleimide has C6-linker that enhances the fluorescence after the dye is conjugated to proteins. The product is a direct replacement for 5(6)-carboxytetramethylrhodamine C5-maleimide (91040).

Fluorescein cadaverine can be coupled to activated carboxy groups or sulfonyl chlorides.

MTS-6-TAMRA (6-carboxytetramethylrhodamine methanethiosulfonate) is a fast-reacting and highly selective thiol-reactive label that permits one to attach the popular fluorescent dye TAMRA to a thiol group.

MTS-5-TAMRA (5-carboxytetramethylrhodamine methanethiosulfonate) is a fast-reacting and highly selective thiol-reactive label that permits one to attach the popular fluorescent dye TAMRA to a thiol group.

DTT (dithiothreitol) is a reducing agent commonly used to reduce cystine disulfides to free thiols.

N-(6-Aminohexyl)maleimide is a cross-linking reagent that can react with an amine-reactive group and a thiol group.

TCEP hydrochloride is an odorless reducing agent that is more stable and effective than dithiothreitol (DTT) or 2-Mercaptoethanol. TCEP hydrochloride is soluble in water to 310 grams per liter.

ABD-F is a useful reagent for the sensitive and specific detection of thiols both by TLC and HPLC (1,2). When combined with a reducing agent such as tributylphosphine, it can also be used for the determination of disulfides in peptides and proteins (3,4).

MTS-sulforhodamine 101 is a fast-reacting and highly selective thiol-reactive rhodamine dye.

3,6,9,12-Tetraoxatetradecane-1,14-diyl dimethanethiosulfonate is a water soluble MTS cross-linker.