New Products
Earth-Friendly Products
Biotium Choice Antibodies
Special Offers
Quick Order Tool
EvaGreen® & EvaGreen® Plus: Next-Gen qPCR Dyes
EvaGreen® dyes are next-generation DNA-binding dyes that are ideal for quantitative real-time PCR (qPCR) and many other applications. Biotium scientists designed the dyes by taking into consideration several essential dye properties relevant to PCR. The result of our efforts is a dye superior to SYBR® Green I and other commercial PCR or high-resolution melt curve (HRM) dyes.*
EvaGreen® Plus Dye is an improved alternative to Biotium’s original EvaGreen® Dye that retains the same essential benefits while providing lower background and higher signal compared to original EvaGreen® Dye. The dye has an excitation maximum that is a better fit for common qPCR instruments, resulting in improved sensitivity. As a result, EvaGreen® Plus Dye can give earlier Ct values and better signal discrimination. These properties offer potential advantages for digital PCR and isothermal amplification applications.
See EvaGreen® Dye Technology to learn more.
*Practicing HRM may require a license from Idaho Technologies, Inc.
EvaGreen® & EvaGreen® Plus
| Product Name | Catalog Number | Size |
|---|---|---|
| EvaGreen® Dye, 20X in Water (trial size) | 31000-T | 1 mL |
| EvaGreen® Dye, 20X in Water | 31000 | 5 x 1 mL |
| EvaGreen® Dye, 2000X in DMSO | 31019 | 50 uL |
| EvaGreen® Plus Dye, 20X in Water (trial size) | 31077-T | 1 mL |
| EvaGreen® Plus Dye, 20X in Water | 31077 | 5 x 1 mL |
EvaGreen®: The only DNA-binding dye validated for ddPCR
See the full list of references here.
Highlighted Citation
“By partitioning each sample prior to thermal cycling, we demonstrate that it is now possible to use a DNA-binding dye for the quantification of multiple target species from a single reaction.”
McDermott et al.
Cheetah™ HotStart Taq
Advantages of Cheetah™ Hotstart Taq
- Rapid 2 minute hot-start activation via novel chemical modification
- Antibody-free, low-DNA Taq for cleaner results
- Superior long-term stability compared to other chemically modified Taq
- Reduced risk of intramolecular cross-linking that can occur in AmpliTaq® Gold
- Convenient, ready-to-use kits and bulk formats are available
Cheetah™ HotStart Taq is available in two different formulations, Cheetah™ FLEX and Cheetah™ PEA-PLEX (see below for more information).
Note: Cheetah™ HotStart Taq is available for licensing and bulk purchase; contact us at techsupport@biotium.com for evaluation samples and to discuss custom size, packaging, or formulation options.
Cheetah™ FLEX HotStart Taq DNA Polymerase
A superior HotStart Taq that is ideal for FLEXible applications
- Validated for fast cycling PCR
- Third-party validated for qPCR-based proximity extension assays (PEA)
- Enzyme available in standard or bulk sizes
Cheetah™ PEA-PLEX HotStart Taq DNA Polymerase
Uniquely formulated for NGS-based high-throughput proximity extension assays (PEA)
- Validated for fast cycling qPCR
- Third-party validated for NGS-based high-throughput PEA
- Enzyme available in standard or bulk sizes
View Product Page
EvaRuby™ Dye for qPCR & HRM® Analysis
A spectrally unique dye to expand multiplexing
EvaRuby™ Dye Features
- Flexible: Unique orange/red DNA-binding dye that can be seamlessly integrated with qPCR probes to expand multiplexing capabilities with ease and precision
- Versatile: Enables the combination of probe-based qPCR with HRM® analysis in a single reaction
- Spectrally Unique: Long Stokes shift and minimal cross-talk in neighboring detection channels, ensuring cleaner and more reliable data
- Convenient: Easily confirm your results with melt curve analysis rather than time consuming post-qPCR gel electrophoresis
View Product Page
Forget-Me-Not™ EvaGreen® qPCR Master Mix
Forget-Me-Not™ qPCR Master Mix is a hot-start EvaGreen® Dye-based master mix for use in real time PCR applications and melt curve analysis. Its color-change system helps ensure that both master mix and template get added to each reaction, so you can catch pipetting mistakes and avoid wasting time, reagents, and your precious DNA samples.
To learn more about our master mixes, please see Forget-Me-Not™ Master Mix Technology.
View Product Page
Forget-Me-Not™ Universal Probe qPCR Master Mix
Forget-Me-Not™ Universal Probe qPCR Master Mix is a high-performance mix for all fluorescent probe-based PCR applications, including quantitation and SNP genotyping. It is suitable for hydrolysis probes and displacement probes, performs well in singleplex and multiplex reactions, has broad instrument compatibility, and can be used in standard and fast protocols.
To learn more about our master mixes, please see Forget-Me-Not™ Master Mix Technology.
View Product Page
N-Flux™ 5X Digital PCR Master Mix
N-Flux™ 5X Digital PCR Master Mix is a high-performance, dye-free mix specially formulated for chip-based digital PCR (cdPCR) platforms. The mix minimizes interactions with hydrophobic plastic surfaces for optimal sample partitioning, resulting in superior separation between positive and negative partitions. N-Flux™ 5X digital PCR Master Mix reproducibly performed genotyping and copy number variation analysis with quantitative discrimination.
- Robust: Sensitive and reproducible performance in dPCR and qPCR
- Optimal partitioning: Reduces interactions with plastic, boosts end-point fluorescence
- Compatible with all fluorescent probe-based technologies: Including TaqMan®, dual-labeled BHQ® probes, and displacement probes
- 5X concentration: Ideal for samples too dilute for 2X master mixes
View Product Page
Full List of Master Mixes
| Catalog No. | Master Mix | EvaGreen® qPCR | Probe-Based PCR | Optimized for dPCR | 1-Color Tracking | 2-Color Tracking |
|---|---|---|---|---|---|---|
| 31041 | Forget-Me-Not™ EvaGreen® qPCR Master Mix with 2-Color Tracking | ✓ | ✓ | |||
| 31042 | Forget-Me-Not™ EvaGreen® qPCR Master Mix with 2-Color Tracking, with separate ROX | ✓ | ✓ | |||
| 31045 | Forget-Me-Not™ EvaGreen® qPCR Master Mix (low ROX) | ✓ | ✓ | |||
| 31046 | Forget-Me-Not™ EvaGreen® qPCR Master Mix (high ROX) | ✓ | ✓ | |||
| 31043 | Forget-Me-Not™ Universal Probe qPCR Master Mix (no ROX) | ✓ | ✓* | |||
| 31044 | Forget-Me-Not™ Universal Probe qPCR Master Mix, with separate ROX | ✓ | ✓* | |||
| 31078 | N-Flux™ 5X Digital PCR Master Mix | ✓ | ✓ |
VeriFluor™ Far-Red Passive Reference Dye
VeriFluor™ Far-Red is a far-red passive reference dye with reliable and steady fluorescent signal for normalizing the reporter dye during PCR. VeriFluor™ acts as a direct replacement for Mustang Purple™ and as an alternative to ROX passive reference dye, allowing more flexibility when designing multiplex qPCR reactions. Unlike Mustang Purple™, VeriFluor™ also has demonstrated insensitivity to common buffer additives, such as DTT.
Features
- Provides a reliable and steady fluorescent signal that enables accurate normalization of qPCR reactions
- Serves as an alternative to ROX, allowing greater flexibility for designing and optimizing multiplexed qPCR assays
- Functions as a direct, drop-in replacement for Mustang Purple™ on most instruments
- Unlike Mustang Purple™, VeriFluor™ is compatible with DTT-containing qPCR reactions
- Exhibits greater solubility than ROX for easier mixing and more accurate results
- Supplied as a stand-alone dye solution for use with our Forget-Me-Not™ Master Mixes or other qPCR master mixes
View Product Page
Eva-EZ™ Polymerase Activity Assay
EvaEZ™ Fluorometric Polymerase Activity Assay Kit provides an easy and accurate way to assess the activity of a polymerase without radioisotopes. This EvaGreen® Dye-based kit exhibits an increased rate of fluorescence that is positively correlated to the activity of polymerase. The assay can be used for Taq, Pfu, Vent, Phusion®, Bst, Phi29, MMLV, AMV, SuperScript®, T4 DNA polymerase, T7 DNA polymerase, Klenow, and E. coli DNA polymerase I.
View Product Page
Viability PCR Dyes & Kits
Biotium is proud to have developed the cutting-edge technology of viability PCR (v-PCR), a technique in which cell viability can be assessed by treating a sample with a dead cell-specific DNA-binding dye, followed by a qPCR or isothermal amplification reaction. We offer two photoreactive, membrane-impermeant, v-PCR dyes (PMA and PMAxx™) that offer superior dead cell selectivity over traditional culture-based methods and the previous EMA v-PCR dye. To learn more about this technology and our portfolio of reagents for viability PCR, please see Viability PCR Technology.
PCR Accessory Reagents & Related Products
| Product Name | Catalog Number | Size |
|---|---|---|
| dNTP Mix, 10 mM Each | 40054 | 5 x 1 mL |
| dNTP Mix, 25 mM Each | 40053 | 1 mL |
| dNTP Set, 100 mM Each | 40052 | 250 uL Each |
| 5-Aminoallyl dUTP (AA-dUTP), 10 mM Solution | 40020 | 100 uL |
| BrdU | 40024 | 100 mg |
| dCTP CF® Dye Conjugates | 9 dye color options | 5 or 25 nmol |
| dUTP CF® Dye Conjugates | 8 dye color options | 5 or 25 nmol |
| ROX Reference Dye | 29052 | 5 x 1 mL |
| EvaGreen® Dye, 2000X in DMSO | 31019 | 50 uL |
| EvaGreen® Dye, 20X in water | 31000 | 5 x 1 mL |
| Cheetah™ Hot-Start Taq Polymerase | 29050 | 500 U |
| HotStart Polymerase Modification Kit | 29054 | 1 kit modifies 0.5 mg of polymerase |
| WarmStart™ Enzyme Modification Kit | 29053 | 1 kit modifies 0.5 mg of protein |
