Exosome-based therapies are gaining attention in regenerative medicine for their ability to modulate cellular behavior and enhance tissue repair. Exosomes derived from mesenchymal stem cells (MSCs) have been shown to carry bioactive molecules such as proteins, lipids, and nucleic acids that facilitate intercellular communication and tissue regeneration. While MSC-derived exosomes have demonstrated therapeutic potential for wound healing, clinical and veterinary exosome therapies remain limited due to challenges in producing sufficient quantities of high-quality exosomes in a scalable and reproducible manner.
In a 2025 Scientific Reports publication, Pamulang et al. addressed these limitations and developed a novel upscaling protocol to isolate exosomes from canine adipose-derived MSCs (cAD-MSCs). This approach aimed to overcome the low exosome yield typical of conventional 2D culture systems while avoiding serum-derived contaminants by combining a microcarrier-based 3D culture system, a custom serum-free medium (VSCBIC-3), and tangential flow filtration. The protocol was evaluated not only for its ability to increase exosome production but also for preserving cell viability and producing vesicles with enhanced bioactivity.
To validate exosome purity and functionality, the team followed MISEV2023 guidelines for comprehensive characterization. Biotium’s AccuOrange™ Protein Quantification Kit was used to measure exosomal protein concentration for determining exosome yield. Expression of the exosomal surface marker CD9 was assessed using Biotium’s ExoBrite™ CD9 Flow Antibody on a CytoFLEX® LX Nanoscale Flow Cytometer. The study validated the scalability and therapeutic potential of cAD-MSC-derived exosomes for wound healing, advancing the potential for exosome therapies in veterinary clinical applications and future translational research.
Learn more about Biotium’s many stains and antibodies for EV research, including new ExoBrite™ CD9/CD63/CD81 Antibody Cocktails for flexible and bright multiplexing detection. Other products in the ExoBrite™ line include ExoBrite™ True EV Membrane Stains, ExoBrite™ EV Total RNA Isolation Kit, and ExoBrite™ STORM Antibodies and CTB EV Stains for super-resolution microscopy with bright and photostable CF® Dyes.
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