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Protein microarrays by 3D nanotexturing

Microarrays have become routine in clinical and environmental testing. This technology enables quick, accurate and reproducible screening of multiple markers simultaneously from minute sample quantities at low cost. Lab-on-a-chip microarray technology constitutes a collection of high-density DNA, protein, or antibody biomolecules (probes) spotted or immobilized on a solid surface. A variety of surfaces have been used as substrates for probe immobilization, but  with limitations including low binding capacity, non-uniform signal intensity and high background.

A recent paper introduces the use of a novel 3D micro-nanotextured polymer substrate to coat the surface of glass slides for high performance protein microarrays that eliminates several of the above issues. In model binding assays using CF®555 dye labeled  IgG and streptavidin, the authors reported that 3D micro-nanotextured microarrays offered significant improvements over epoxy-coated glass slides in terms of spot intensity, homogeneity, and reproducibility, with better signal to noise ratio values over a wide range of protein concentrations. The coated slides also had a shelf-life over one year without requirement for specific storage conditions.

MCF-7 cells stained with rabbit anti-COXIV and CF®555 goat anti-rabbit (mitochondria, red) and mouse anti-ZO1 and CF®647 goat anti-mouse (tight junctions, green) and mounted in EverBrite™ Mounting Medium with DAPI.

Learn more about Biotium’s bright and photostable CF® dyes, and CF® dye labeled antibodies and bioconjugates