Biotium Expands Tyramide Signal Amplification Portfolio with Brighter and More Stable Dyes for Enhanced Spatial Imaging

Biotium’s new TyraMax™ Amplification Dyes and Kits are available with the widest selection of colors for spatial analysis.

Fremont, CA — November 14, 2025  — Biotium, a leading innovator in life science reagents, is excited to introduce TyraMax™ Amplification Dyes and Kits. Designed for superior brightness, photostability, and stability in amplification buffer compared to competitor reagents, TyraMax™ dyes deliver the best performance in tyramide signal amplification (TSA) for spatial biology and multiplex fluorescence imaging.

The TyraMax™ line offers the widest selection of dye colors on the market, with unique options spanning from blue to near-infrared for unmatched multiplexing flexibility and spectral panel design. All TyraMax™ dyes, including near-IR dyes, are chemically stable in amplification buffer for at least 24 hours. This makes them ideal for automated staining workflows in spatial biology platforms with more resistance to oxidation unlike other TSA dyes. Compared to conventional immunofluorescence, TyraMax™ Dyes provide up to 100-fold higher detection sensitivity when used with TSA while maintaining sharp, photostable signals for multiplex imaging workflows like cyclic immunofluorescence (CycIF).

“Advances in spatial analysis are expanding our understanding of complex biology, yet many methods still depend on underperforming traditional probes. Our next-generation TyraMax™ dyes complement these innovations with improved performance and panel flexibility for researchers.” -Lori Roberts, PhD, Director of Bioscience.

Signal Stability You Can Count On

TyraMax™ 740/770 offers excellent brightness, photostability, and working solution stability, making it ideal for automated workflows. PFA-fixed HeLa cells stained with WGA-HRP were detected using TyraMax™ 740/770 or Aluora™ 750 and imaged in PBS on an Evident FV4000 confocal microscope (40X oil, 730 nm laser, Alexa Fluor® 700 channel). For photostability, fluorescence was recorded every second under continuous 10% laser illumination and normalized to the initial intensity. For stability testing, OD-matched dye solutions in Tyramide Amplification Buffer Plus (0.0015% H₂O₂) were monitored by absorbance over 24 hours at room temperature. TyraMax™ 740/770 remained stable, while Aluora™ 750 degraded almost completely.

Key Features:

• Largest selection of tyramide dye colors available, from blue to near-IR
• Stable in amplification buffer for at least 24 hours, including near-IR dyes
• Brighter and more photostable than competitor TSA dyes
• Available as standalone dyes, trial sizes, or multiplex dye sets
• Compatible with automated workflows using Biotium’s Tyramide Amplification Buffer Plus

For maximum flexibility, Biotium offers several ready-to-use dye set options for custom panel development or large-scale experiments:

• TyraMax™ 3-Plex Amplification Dye Set with DAPI (Cat. No. 33029)
• TyraMax™ 5-Plex Amplification Dye Set with DAPI (Cat. No. 33030)
• TyraMax™ Amplification Dye Spectral Sampler (Cat. No. 33031)

TyraMax™ Dyes outperform leading competitors such as Thermo Fisher’s Aluora® Spatial Dyes and Opal® Polaris from Akoya Biosciences in brightness and stability at a more accessible price point.

Biotium’s TyraMax™ line empowers researchers with the confidence to build and optimize complex panels for spatial omics, tissue mapping, and other cutting-edge imaging applications. TyraMax™ Dyes expand Biotium’s innovative line of tools for Tyramide Signal Amplification, and are available through Biotium and authorized distributors worldwide.

Aluora and Alexa Fluor are registered trademarks of Thermo Fisher Scientific; Opal is a registered trademark of Akoya Biosciences.