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Viability/Cytotoxicity Assay Kit for Bacteria Live & Dead Cells

This kit provides two-color fluorescence staining of both live bacteria (green) and dead bacteria (red) using two DNA dyes, DMAO and EthD-III.

Product Attributes

Apoptosis/viability marker

Live/dead assay

For live or fixed cells

For live/intact cells

Detection method/readout

Fluorescence microscopy, Flow cytometry

Assay type/options

Endpoint assay, No-wash staining, Real-time imaging, Homogenous assay

Colors

Green/Red

Size
Catalog #
price
Qty
100-1000 assays
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Product Description

This kit provides two-color fluorescence staining of both live bacteria (green) and dead bacteria (red) using two DNA probes, DMAO and EthD-III.

Features

  • Detect live and dead bacteria cells in the same cell population
  • Simple, rapid no-wash staining
  • For fluorescence microscopy or flow cytometry

Kit Components

  • DMAO, 5 mM solution (2 x 100 uL)
  • EthD-III, 2 mM solution (2 x 150 uL)

Spectral properties

  • EthD-III: Ex/Em 279, 522/593 nm (with DNA)
  • DMAO: Ex/Em 496/528 nm (with DNA)

DMAO is a green-fluorescent nucleic acid dye which stains both live and dead bacteria. Ethidium Homodimer III (EthD-III) is a red-fluorescent nucleic acid dye that selectively stains dead bacteria with damaged cell membranes. When stained with a mixture of DMAO and EthD-III, bacteria with intact cell membranes show green fluorescence, whereas bacteria with damaged cell membranes show red fluorescence. The kit is suitable for use with fluorescence microscopes and flow cytometers. The assay principles are general and applicable to most bacteria types. For 100 flow cytometry assays or 1000 microscopy assays.

See our full selection of Microbiology Stains & Kits.

References

1. J Marine Sci Res Dev (2011) S5, 001. doi:10.4172/2155-9910.S5-001
2. PLoS Genet (2012) 8(1): e1002459. doi:10.1371/journal.pgen.1002459
3. Artificial Cells Nanomed Biotech (2013) 41(2), 116-124. DOI: 10.3109/10731199.2012.712047
4. Appl Environ Microbiol (2015) 81, 6757–6766. doi:10.1128/AEM.01953-15
5. ACS Appl Mater Interfaces (2016) 8(14), 8928-8938. https://doi.org/10.1021/acsami.6b00093
6. Sci Rep (2015) 5, 15681. DOI: 10.1038/srep15681
7. Polish J Microbiol (2019) 68(1), 71–81. https://doi.org/10.21307/pjm-2019-009

 

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