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Viability/Cytotoxicity Assay Kit for Bacteria Live & Dead Cells

This kit provides two-color fluorescence staining of both live bacteria (green) and dead bacteria (red) using two DNA dyes, DMAO and EthD-III.

Product Attributes

Apoptosis/viability marker

Live/dead assay

For live or fixed cells

For live/intact cells

Detection method/readout

Fluorescence microscopy, Flow cytometry

Assay type/options

Endpoint assay, Homogeneous assay, No-wash staining, Real-time imaging



Storage Conditions

Store at 2 to 8 °C, Protect from light

Catalog #
1000 assays
Clear selection
(Please select quantity above )

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Product Description

This kit provides two-color fluorescence staining of both live bacteria (green) and dead bacteria (red) using two DNA probes, DMAO and EthD-III.


  • Detect live and dead bacteria cells in the same cell population
  • Simple, rapid no-wash staining
  • For fluorescence microscopy or flow cytometry

Kit Components

  • DMAO, 5 mM solution (2 x 100 uL)
  • EthD-III, 2 mM solution (2 x 150 uL)

Spectral properties

  • EthD-III: Ex/Em 279, 532/625 nm (with DNA)
  • DMAO: Ex/Em 496/528 nm (with DNA)

DMAO is a green-fluorescent nucleic acid dye which stains both live and dead bacteria. Ethidium Homodimer III (EthD-III) is a red-fluorescent nucleic acid dye that selectively stains dead bacteria with damaged cell membranes. When stained with a mixture of DMAO and EthD-III, bacteria with intact cell membranes show green fluorescence, whereas bacteria with damaged cell membranes show red fluorescence. The kit is suitable for use with fluorescence microscopes and flow cytometers. The assay principles are general and applicable to most bacteria types. For 100 flow cytometry assays or 1000 microscopy assays.

Biotium also offers BactoView™ Dead Stains and BactoView™ Viability Kits for highly-selective staining of dead bacteria, available in a selection of 7 colors from green to near-IR. BactoView™ Dead Stains also can be combined with fluorescent Gram stains like our CF® Dye WGA Conjugates.

BactoView™ Live Green and BactoView™ Live Red are designed for staining both live and dead bacteria. Note that BactoView™ Stains cannot be used to distinguish bacteria from eukaryotic cells, because they will stain other cell types as well. For bright and optimized labeling of bacterial endospores, see our BactoSpore™ Bacterial Stains.

For staining mammalian cells, see our NucSpot® Nuclear Stains for live/dead discrimination or nuclear counterstaining of fixed mammalian cells. For live nuclear staining of mammalian cells, see our NucSpot® Live Stains. Also, view our Cellular Stains Table for more information on how our dyes stain various organisms.

See our full selection of Microbiology Stains & Kits.


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