Support

Quick links
Frequently asked questions - categories
Ask a question
Contact Us
+1

Calcein (High purity)

Calcein is a highly negatively charged, water-soluble fluorescein derivative. Its fluorescence is nearly independent of pH in the range between 6.5 and 12.

Product Attributes

CAS number

154071-48-4

Cellular localization

Fluid phase tracer

Cell permeability

Membrane impermeant

Colors

Green

Certificates of Analysis (COA)

Request COA
Size
Catalog #
price
Qty
100 mg
Clear selection
Add to wishlist
ADD TO CART
(Please select quantity above )

Wishlist updated! View wishlist

Shipping, Handling & Tax (CA only) not included in price. Learn more

Tax is assessed in the state of California. All fees will be calculated during checkout based on your shipping information and are subject to change.

SPECIAL - Order 5 or more and receive 10% off.

Request a Bulk Quote

Product Description

Calcein is a highly negatively charged, water-soluble fluorescein derivative. Its fluorescence is nearly independent of pH in the range between 6.5 and 12. The dye can be introduced into cells by microinjection and is useful for cell tracing and for the study of cell membrane integrity and fusion. Biotium also offers calcein AM, the membrane-permeant version of calcein that hydrolyzes into calcein once inside cells.

  • λEx/λEm = 494/517 nm (pH 8).
  • ε = 75,000
  • Yellow-orange solid soluble in DMSO, DMF or pH>6 water
  • Store at 4°C and protect from light
  • C30H26N2O13
  • MW: 623
  • [154071-48-4]

 

You may also like…

    Calcein AM

    Calcein AM is a widely used green fluorescent cell marker. Calcein AM is membrane-permeant and thus can be introduced into cells via incubation.

    Calcein AM Cell Viability Assay Kit

    The Calcein AM Cell Viability Assay Kit is designed to quantify live cell numbers based on their endogenous esterase activity and plasma membrane integrity.

    Viability/Cytotoxicity Assay Kit for Animal Live & Dead Cells

    The Viability/Cytotoxicity Assay Kit for Animal Live & Dead Cells provides green/red fluorescent staining of live and dead cells, respectively. The assay employs two probes that detect intracellular esterase activity in live cells and compromised plasma membrane integrity in dead cells.

    CF® Dye Hydrazide

    Our CF® dye hydrazides are bright, extremely water-soluble and nontoxic. These properties make the dyes excellent fixable polar tracers for neurons, or for gap junction studies.

    CF® Dye Biotin

    Potential applications of Biotin-CF® dye include detection of biotin binding sites and the degree of biotinylation of proteins, and for the measurement of avidin and streptavidin in crude biological samples. In addition, biotin-CF® dye can be used as a polar tracer by microinjection to study the morphology of cells.

    CF® Dye Dextran 10,000 MW, Anionic and Fixable

    CF® dye labeled dextrans could be used as a fluorescent fluid-phase markers to study cell permeability, endocytosis, or mechanisms of biomolecular delivery. The dextran is 10,000 MW, and contains a formaldehyde-fixable free-amine group.