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CD63 Monoclonal Mouse Antibody (H5C6) – Biotium Choice

CD63 (clone H5C6) is a validated mouse monoclonal antibody that recognizes the human tetraspanin protein CD63. The antibody belongs to the Biotium Choice list of select antibodies that have been validated and optimized in-house for optimal performance.

Product Attributes

Antibody number




Biotium Choice Antibody


Antibody type

Biotium Choice Flow Antibodies, Primary



Host species





IgG1, kappa

Antibody reactivity (target)



gp55; granulophysin; Lysosomal-associated membrane protein 3 (LAMP-3); Mast cell antigen AD1; melanoma 1 antigen; Melanoma-associated antigen MLA1; Melanoma-associated antigen ME491; MLA1; NGA; Ocular melanoma-associated antigen; OMA81H; PTLGP40; Tetraspanin-30; TSPAN30

Species reactivity

Baboon, Cynomolgus monkey, Human, Non-human primates

Human gene symbol


Entrez gene ID




Molecular weight

26 kDa (core protein); 30-60 kDa (glycosylated)

Antibody target cellular localization

Exosomes/EVs, Lysosomes, Plasma membrane, Membrane/vesicular, Multivesicular bodies

Cell/tissue expression

Exosomes, Platelets, Granulocytes, Lymphocytes, Monocytes/macrophages

Verified antibody applications

Flow (surface) (verified), IF (verified), WB (verified)

Positive control

U87MG, SK-MEL-28, HL60, THP-1, NIH/3T3, or MCF-7 cells. Human melanoma, spleen or lymphoma tissue.

Antibody application notes

Flow Cytometry: 0.5-1 ug/mL (or, 0.05-0.1 ug/million cells), Immunofluorescence: 1 ug/mL, Western Blot: 0.1 ug/mL

Antibody research areas


Antibody/conjugate formulation

Purified: 0.5 mg/mL in PBS/0.05% azide, Conjugates: PBS/0.1% BSA/0.05% azide

Shelf life

Guaranteed for at least 24 months from date of receipt when stored as recommended

Volume per assay

5 uL/test

Storage Conditions

Store at 2 to 8 °C

Regulatory status

For research use only (RUO)

Catalog #
25 tests
100 tests
200 uL
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Product Description

CD63 (clone H5C6) is a mouse monoclonal antibody that recognizes the human tetraspanin protein CD63. This antibody belongs to the Biotium Choice list of select antibodies that have been validated and optimized in-house for optimal performance. This antibody has been validated in flow cytometry, in immunofluorescence microscopy, and in western blot. This antibody is available conjugated to R-Phycoerythrin (R-PE or PE). It is supplied in PBS, 0.1% BSA, 0.05% azide or purified at 0.5 mg/mL, BSA-free.

The tetraspanins are integral membrane proteins expressed on cell surface and granular membranes of hematopoietic cells and are components of multi-molecular complexes with specific integrins. The tetraspanin CD63 is a lysosomal membrane glycoprotein that translocates to the plasma membrane after platelet activation. CD63 is expressed on activated platelets, monocytes and macrophages, and is weakly expressed on granulocytes, T cell and B cells. It is located on the basophilic granule membranes and on the plasma membranes of lymphocytes and granulocytes. CD63 is a member of the TM4 superfamily of leukocyte glycoproteins that includes CD9, CD37 and CD53, which contain four transmembrane regions. CD63 may play a role in phagocytic and intracellular lysosome-phagosome fusion events. CD63 deficiency is associated with Hermansky-Pudlak syndrome and is strongly expressed during the early stages of melanoma progression.

Tetraspanin proteins CD9, CD63, and CD81, are the most common proteins used as exosome markers. Tetraspanins are plasma membrane proteins with many proposed functions, including activation and sorting of other membrane proteins. They are also thought to play a role in the targeting of proteins to multivesicular bodies (MVBs) and exosomes. These tetraspanins are broadly expressed on many cell types and can therefore be detected on many types of exosomes, but their expression levels vary depending on the cell type of origin.

As an isotype control, we recommend Isotype Control, Monoclonal Mouse IgG1 Kappa (IGG1/1331) for this antibody. View our full catalog of Biotium Choice antibodies, optimized and validated for the best results in flow cytometry.

Exosome Stains and Antibodies

Biotium also offers ExoBrite™ CD63 Flow Antibody and ExoBrite™ CD63 Western Antibody that are validated for optimal detection of isolated exosomes by flow cytometry or western blotting. Learn more about our products for exosome research.

CD63 Monoclonal Mouse Antibody (H5C6) - Biotium Choice

ConjugationEx/EmConc.SizeCatalog No.Availability
CF®405M416/452 nm100 ug/mL25 tests (125 uL)P004S-405M-125Discontinued, see replacement P013-405M
100 tests (500 uL)P004S-405M-500
CF®488A490/516 nm100 ug/mL25 tests (125 uL)P004S-488A-125Discontinued, see replacement P013-488A
100 tests (500 uL)P004S-488A-500
CF®568562/584 nm100 ug/mL25 tests (125 uL)P004S-568-125Discontinued, see replacement P013-568
100 tests (500 uL)P004S-568-500
CF®700696/721 nm100 ug/mL25 tests (125 uL)P004S-700-125Discontinued, see replacement P013-700
100 tests (500 uL)P004S-700-500
R-PE496, 546, 565/576 nm100 ug/mL25 tests (125 uL)P004S-RPE-125Available
100 tests (500 uL)P004S-RPE-500
Purified (BSA-free)N/A0.5 mg/mL200 uL (100 ug)P004-200uLAvailable

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