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CD81 Monoclonal Mouse Antibody (5A6)

CD81 (clone 5A6) is a validated mouse monoclonal antibody that recognizes the human tetraspanin protein CD81.

Product Attributes

Antibody number

#P005

Conjugation

Purified (BSA-free, 0.5 mg/mL)

Storage Conditions

Store at 2 to 8 °C

Shelf life

Guaranteed for at least 24 months from date of receipt when stored as recommended

Antibody/conjugate formulation

Purified: 0.5 mg/mL in PBS/0.05% azide

Antibody research areas

Exosomes/EVs

Antibody application notes

Flow Cytometry: 0.5-1 ug/mL (or, 0.05-0.1 ug/million cells), Immunofluorescence: 1 ug/mL, Western Blot: 0.1 ug/mL

Positive control

Ramos, Daudi, Raji, U266, HUT-78, HUT-102, Jurkat, HL-60, KG1a, A293, A431, MG63, MCF-7, and human lymphocytes. Tonsil or liver tissue.

Verified antibody applications

Flow (surface) (verified), IF (verified), WB (verified)

Cell/tissue expression

Exosomes, Hepatocytes, Lymphocytes, Monocytes/macrophages

Antibody target cellular localization

Exosomes/EVs, Plasma membrane

Molecular weight

26 kDa

SwissProt

P60033

Unigene

54457

Entrez gene ID

975

Human gene symbol

CD81

Species reactivity

Baboon, Cynomolgus monkey, Human, Non-human primates

Synonyms

CD81; Target of the antiproliferative antibody 1 (TAPA-1); Tetraspanin-28; Tspan-28

Antibody reactivity (target)

CD81

Isotype

IgG1, kappa

Clone

5A6

Host species

Mouse

Clonality

Monoclonal

Antibody type

Primary

Regulatory status

For research use only (RUO)

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200 uL
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Product Description

CD81 (clone 5A6) is a mouse monoclonal antibody that recognizes the human tetraspanin protein CD81. This antibody has been validated in flow cytometry, in immunofluorescence microscopy, and in western blot. It is supplied at 0.5 mg/mL, BSA-free.

CD81 has a very broad cellular distribution, being expressed on T- and B-lymphocytes, NK cells, thymocytes, eosinophils, fibroblasts, epithelial and endothelial cells. Neutrophils, erythrocytes and platelets are negative, while monocytes are variably positive. CD81 is a member of a family of tetraspanin transmembrane proteins, including CD9, CD37, CD53, CD63, and CD82. It associates with CD19, CD21, Leu 13, and integrins on cell membrane and is involved in signal transduction in B lymphocyte development and cell adhesion. CD81 also acts as a receptor for the envelope protein E2 of chronic hepatitis C virus. Antibodies to CD81 have anti-proliferative effects on different lymphoid cell lines, particularly those derived from large cell lymphomas.

Tetraspanin proteins CD9, CD63, and CD81, are the most common proteins used as exosome markers. Tetraspanins are plasma membrane proteins with many proposed functions, including activation and sorting of other membrane proteins. They are also thought to play a role in the targeting of proteins to multivesicular bodies (MVBs) and exosomes. These tetraspanins are broadly expressed on many cell types and can therefore be detected on many types of exosomes, but their expression levels vary depending on the cell type of origin.

Exosome Stains and Antibodies

Biotium also offers ExoBrite™ CD81 Flow Antibody and ExoBrite™ CD81 Western Antibody that are validated for optimal detection of isolated exosomes by flow cytometry or western blotting. Learn more about our products for exosome research.

 

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