The AccuClear® Ultra High Sensitivity dsDNA Quantitation Kit offers sensitive and selective detection of purified DNA samples, designed for use with fluorescence microplate readers. AccuClear® is our most popular DNA quantitation kit, with a very wide linear detection range from 0.03 – 250 ng. The AccuClear® assay is also available without any DNA standards (31027), for those that wish to use their own standard.
- The most versatile DNA quantitation kit
- Extremely wide linear range of 0.03-250 ng DNA
- Highly specific for dsDNA
- Designed for fluorescence microplate readers
- Green fluorescence emission
- AccuClear® Dye, 100X
- DNA Quantitation Buffer, 20X Concentrate*
- dsDNA Standard, 25 ng/uL (in 2000 assay & trial size kits)
- dsDNA Standards, Set of 7 (in 1000 assay kit only)
*Note: In order to go green and save on packaging and shipping, we have changed from a 1X to a 20X buffer.
Fluorescence-Based dsDNA Quantitation
AccuBlue®, AccuClear® and AccuGreen™ dsDNA quantitation assays allow precise quantitation of purified dsDNA samples across a wide range of concentrations and a variety of fluorescence detection instruments. Unlike absorbance-based nucleic acid quantitation, fluorescent DNA binding dyes are highly sensitive and selective for double-stranded DNA and provide a more accurate DNA concentration in the presence of contaminating RNA and other common contaminants including free nucleotides, protein, detergents and salts.
Biotium offers dsDNA quantitiation kits and solutions for different instruments and sample concentration ranges. See the table below and visit the DNA & RNA Quantitation Technology Page for details on our full line of dsDNA and RNA quantitation kits.
All DNA Quantitation Kits
|dsDNA Quantitation Kit||Linear detection range|
(dsDNA in assay)
|Ex/Em (nm)||Suggested Instrument||Features
| AccuGreen High Sensitivity||0.1-100 ng||502/523||Qubit® fluorometer||Compare to the Qubit® dsDNA HS assay from Thermo Fisher
| AccuGreen Broad Range||2-1000 ng||500/530||Qubit® fluorometer||Compare to the Qubit® dsDNA BR assay from Thermo Fisher
Non-toxic & non-mutagentic DNA quantitation dye
| AccuBlue® NextGen||1 pg-3 ng*||468/507||Fluorescent Plate Reader||Most sensitive assay available for quantitation of precious or dilute samples
Optimal for sensitive applications such as NGS or digital PCR
* Limit of detection in the range of 1 pg to 5 pg depending on instrument sensitivity
| AccuClear® Ultra High Sensitivity||30 pg-250 ng||468/507||Fluorescent Plate Reader||Versatile kit with high sensitivity and wide linear range
| AccuBlue® High Sensitivity||0.2-100 ng||485/530||Fluorescent Plate Reader||Non-toxic & non-mutagentic DNA quantitation dye
| AccuBlue® Broad Range||2-2000 ng||350/460||Fluorescent Plate Reader||Broad linear range with blue fluorescence
AccuBlue and AccuClear are registered trademarks of Biotium, Inc. AccuBlue, AccuClear and AccuGreen technologies are covered by granted U.S. and international patents. Qubit is a registered trademark of Thermo Fisher Scientific.