Support & Resources

+1

AccuClear® Ultra High Sensitivity dsDNA Quantitation Kit with DNA Standards

A fluorescent dsDNA quantitation kit with a wide linear range of 0.03-250 ng DNA. Designed for use in a fluorescence microplate reader.

Product Attributes

Excitation/Emission

468/507 nm (with DNA)

Storage Conditions

Store at 2 to 8 °C

Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
Size
Catalog #
price
Qty
Trial size (200 assays)
1,000 assays
2,000 assays
Clear selection
Add to wishlist
ADD TO CART
(Please select quantity above )

Wishlist updated! View wishlist

Shipping, Handling & Tax (CA only) not included in price. Learn more

Tax is assessed in the state of California. All fees will be calculated during checkout based on your shipping information and are subject to change.

SPECIAL - Order 5 or more and receive 10% off.

Request a Bulk Quote

Product Description

The AccuClear® Ultra High Sensitivity dsDNA Quantitation Kit offers sensitive and selective detection of purified DNA samples, designed for use with fluorescence microplate readers. AccuClear® is our most popular DNA quantitation kit, with a very wide linear detection range from 0.03 – 250 ng. The AccuClear® assay is also available without any DNA standards (31027), for those that wish to use their own standard.

Features

  • The most versatile DNA quantitation kit
  • Extremely wide linear range of 0.03-250 ng DNA
  • Highly specific for dsDNA
  • Designed for fluorescence microplate readers
  • Green fluorescence emission

Kit Components

  • AccuClear® Dye, 100X
  • DNA Quantitation Buffer, 20X Concentrate*
  • dsDNA Standard, 25 ng/uL (in 2000 assay & trial size kits)
  • dsDNA Standards, Set of 7 (in 1000 assay kit only)

*Note: In order to go green and save on packaging and shipping, we have changed from a 1X to a 20X buffer.

Fluorescence-Based dsDNA Quantitation

AccuBlue®, AccuClear® and AccuGreen™ dsDNA quantitation assays allow precise quantitation of purified dsDNA samples across a wide range of concentrations and a variety of fluorescence detection instruments. Unlike absorbance-based nucleic acid quantitation, fluorescent DNA binding dyes are highly sensitive and selective for double-stranded DNA and provide a more accurate DNA concentration in the presence of contaminating RNA and other common contaminants including free nucleotides, protein, detergents and salts.

Biotium offers dsDNA quantitiation kits and solutions for different instruments and sample concentration ranges. See the table below and visit the DNA & RNA Quantitation Technology Page for details on our full line of dsDNA and RNA quantitation kits.

All DNA & RNA Quantitation Kits

Kit DNA
or RNA
Detection range
(in assay)*
Dye Ex/Em (nm)Suggested instrumentFeatures
AccuGreen™
High Sensitivity DNA
DNA0.1-100 ng502/523Qubit® fluorometerCompare to the Qubit® dsDNA HS assay from Thermo Fisher
AccuGreen™ Broad Range DNADNA2-1000 ng500/530Qubit® fluorometerCompare to the Qubit® dsDNA BR assay from Thermo Fisher
Non-toxic & non-mutagentic DNA quantitation dye
AccuBlue® NextGen DNADNA1-3000 pg**468/507Fluorescence microplate readerMost sensitive assay available for quantitation of precious or dilute samples
Optimal for sensitive applications such as NGS or digital PCR
AccuClear® Ultra High Sensitivity DNADNA0.03-250 ng468/507Fluorescence microplate readerVersatile kit with high sensitivity and wide linear range
AccuBlue® High Sensitivity DNADNA0.2-100 ng485/530Fluorescence microplate readerNon-toxic & non-mutagentic DNA quantitation dye
AccuBlue® Broad Range DNADNA2-2000 ng350/460Fluorescence microplate readerBroad linear range with blue fluorescence
AccuBlue® Broad Range RNARNA5-1000 ng650/670Fluorescence microplate reader
or Qubit® fluorometer
The widest linear range of available RNA quantitation kits
Exceptional accuracy, sensitivity, and high RNA selectivity
* Standard assay volume is 200 uL
** AccuBlue® NextGen limit of detection is in the range of 1 pg to 5 pg depending on instrument sensitivity
AccuBlue and AccuClear are registered trademarks of Biotium, Inc. AccuBlue, AccuClear and AccuGreen technologies are covered by granted U.S. and international patents. Qubit is a registered trademark of Thermo Fisher Scientific.

References

1. J Food Sci (2015) May;80(5):M1031-8 doi: 10.1111/1750-3841.12848
2. Analytical Biochemistry (2016) 15 Oct pp74-79 doi:10.1016/j.ab.2016.07.022
3. Microbiome (2018), Oct 20;6(1):188 doi:10.1186/s40168-018-0529-x
4. Biotechniques (2018) 64:59-68 doi:10.4155/btn-2017-0102

 

You may also like…