The AccuBlue™ NextGen dsDNA Quantitation Kit provides an unprecedented level of sensitivity in dsDNA detection. The AccuBlue™ NextGen dsDNA Quantitation assay is the most sensitive and accurate DNA quantitation method for ultra low amounts of DNA. With a detection limit as low as 1 pg DNA in a 96-well plate reader assay (see Table 1), AccuBlue™ NextGen outperforms rivals such as PicoGreen® in sensitivity and accuracy. Specially designed for scientists who need to quantify extremely dilute or precious DNA, AccuBlue™ NextGen is ideal for use in preparing samples for applications such as Next Generation Sequencing (NGS) and digital PCR.
The AccuBlue™ NextGen dsDNA quantitation assay is designed for use with fluorescence 96-well plate readers equipped with excitation and emission filters for detecting green fluorescence. The unique spectral properties of AccuBlue™ NextGen Dye make it especially well-suited for use with instruments with blue LED excitation sources. Because the AccuBlue™ NextGen assay is so sensitive, results will vary depending on many factors, such as microplate reader (see Table 1), assay volume, and pipet accuracy.
The AccuBlue™ NextGen dsDNA Quantitation Kit contains AccuBlue™ NextGen Dye, Enhancer, assay buffer, and dsDNA standard, with instructions for preparing a set of standards by serial dilution.
- AccuBlue™ NextGen Dye, 400X
- AccuBlue™ NextGen Enhancer, 100X
- AccuBlue™ NextGen Buffer, 1X
- High Sensitivity dsDNA Standard *, 10 ng/uL
*Note: The concentration of the DNA Standard included in this kit (10 ng/uL) is different than the concentration of the DNA Standard (300 pg/uL) included in the previous version of this kit. The standard was changed due to DNA adsorption issues.
- Linear range: 1-3000 pg dsDNA
- Green fluorescence (Ex/Em 460/507 nm)
- Unrivaled sensitivity (see Ref. 1)
- Assay stability: prepared working solution can be stored for up to 6 months in the fridge for added convenience.
- Green fluorescent dye is a perfect match for blue LED excitation sources
- Green fluorescence detection compatible with fluorescence microplate readers, handheld fluorometers, and NanoDrop® fluorospectrophotometers.
|reader||assay format (assay volume)||lowest DNA detected|
|BMG CLARIOStar||96-well (200 uL)||1 pg|
|BMG CLARIOStar||384-well (50 uL)||0.6 pg|
|Biotek Synergy H1m||96-well (200 uL)||5 pg|
|SpectraMax Gemini XS||96-well (200 uL)||5 pg|
|SpectraMax i3||384-well (50 uL)||1.25 pg|
Other DNA Quantitation Kits:
|dsDNA Quantitation Kit||Linear detection range (dsDNA in assay)||Ex/Em (nm)||Features|
|AccuClear Ultra High Sensitivity||30 pg-250 ng||468/507||This kit offers great sensitivity and the widest linear range.
|AccuBlue Broad Range||2-2000 ng||350/460||For quantitation of high concentration DNA in a broad linear range. Assay can be extended to 4000 ng with minor loss of linearity.
|AccuBlue NextGen||1 pg*-3 ng||468/507||Quantitation of extremely precious or dilute samples. Optimal for sensitive applications such as NGS or digital PCR. *Limit of detection in the range of 1 pg to 5 pg depending on instrument sensitivity.
|AccuBlue High Sensitivity||0.2-100 ng||485/530|
|AccuGreen High Sensitivity||0.1-100 ng||502/523||Quantitation of DNA using the Qubit fluorometer from Thermo Fisher.|
AccuBlue NextGen technology is covered by U.S. patents.