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AccuBlue® NextGen dsDNA Quantitation Kit

The AccuBlue® NextGen dsDNA Quantitation Kit provides an unprecedented level of sensitivity in dsDNA detection. The AccuBlue® NextGen dsDNA Quantitation assay is the most sensitive and accurate DNA quantitation method for ultra low amounts of DNA.

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Product Description

The AccuBlue® NextGen dsDNA Quantitation Kit provides an unprecedented level of sensitivity in dsDNA detection. The AccuBlue® NextGen dsDNA Quantitation assay is the most sensitive and accurate DNA quantitation method for ultra low amounts of DNA. With a detection limit as low as 1 pg DNA in a 96-well plate reader assay (see Table 1), AccuBlue® NextGen outperforms rivals such as PicoGreen® in sensitivity and accuracy. Specially designed for scientists who need to quantify extremely dilute or precious DNA, AccuBlue® NextGen is ideal for use in preparing samples for applications such as Next Generation Sequencing (NGS) and digital PCR.

The AccuBlue® NextGen dsDNA Quantitation Kit contains a 10 ng/uL dsDNA standard, with instructions for preparing a set of standards by serial dilution. We also offer the AccuBlue® NextGen dsDNA Quantitation Solution without DNA standard (catalog no. 31061), for customers who wish to use their own standard.

The AccuBlue® NextGen dsDNA quantitation assay is designed for use with fluorescence 96-well plate readers equipped with excitation and emission filters for detecting green fluorescence. The unique spectral properties of AccuBlue® NextGen Dye make it especially well-suited for use with instruments with blue LED excitation sources. Because the AccuBlue® NextGen assay is so sensitive, results will vary depending on many factors, such as microplate reader (see table below), assay volume, and pipet accuracy.

For DNA quantitation using the Qubit® fluorometer we recommend our AccuGreen™ High Sensitivity dsDNA Quantitation Kit (catalog no. 31066) and AccuGreen™ Broad Range dsDNA Quantitation Kit (catalog no. 31069), which were formulated specifically for that instrument.

Features

  • Linear range: 1-3000 pg dsDNA
  • Unrivaled sensitivity
  • Green fluorescent dye is a perfect match for blue LED excitation sources

Components

  • AccuBlue® NextGen Dye, 400X
  • AccuBlue® NextGen Enhancer, 100X
  • AccuBlue® NextGen Buffer, 1X
  • High Sensitivity DNA Standard, 10 ng/uL

Plate Reader Detection Limits

Plate readerAssay format (assay volume)Lowest DNA detected
BMG CLARIOStar96-well (200 uL)1 pg
BMG CLARIOStar384-well (50 uL)0.6 pg
Biotek Synergy H1m96-well (200 uL)5 pg
SpectraMax Gemini XS96-well (200 uL)5 pg
SpectraMax i3384-well (50 uL)1.25 pg

All DNA Quantitation Kits

dsDNA Quantitation KitLinear detection range
(dsDNA in assay)
Ex/Em (nm)Suggested InstrumentFeatures
AccuGreen™ High Sensitivity0.1-100 ng502/523Qubit® fluorometerCompare to the Qubit® dsDNA HS assay from Thermo Fisher
AccuGreen™ Broad Range2-1000 ng500/530Qubit® fluorometerCompare to the Qubit® dsDNA BR assay from Thermo Fisher
Non-toxic & non-mutagentic DNA quantitation dye
AccuBlue® NextGen1 pg-3 ng*468/507Fluorescent Plate ReaderMost sensitive assay available for quantitation of precious or dilute samples
Optimal for sensitive applications such as NGS or digital PCR
* Limit of detection in the range of 1 pg to 5 pg depending on instrument sensitivity
AccuClear® Ultra High Sensitivity30 pg-250 ng468/507Fluorescent Plate ReaderVersatile kit with high sensitivity and wide linear range
AccuBlue® High Sensitivity0.2-100 ng485/530Fluorescent Plate ReaderNon-toxic & non-mutagentic DNA quantitation dye
AccuBlue® Broad Range2-2000 ng350/460Fluorescent Plate ReaderBroad linear range with blue fluorescence

AccuBlue and AccuClear are registered trademarks of Biotium, Inc. AccuBlue, AccuClear and AccuGreen technologies are covered by granted U.S. and international patents. Qubit and Quant-iT are registered trademarks of Thermo Fisher Scientific.

References

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