AccuBlue NextGen dsDNA Quantitation Kit

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Catalog #: 31060, 31060-T
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Product Description

The AccuBlue™ NextGen dsDNA Quantitation Kit provides an unprecedented level of sensitivity in dsDNA detection. The AccuBlue™ NextGen dsDNA Quantitation assay is the most sensitive and accurate DNA quantitation method for ultra low amounts of DNA. With a detection limit as low as 1 pg DNA in a 96-well plate reader assay (see Table 1), AccuBlue™ NextGen outperforms rivals such as PicoGreen® in sensitivity and accuracy. Specially designed for scientists who need to quantify extremely dilute or precious DNA, AccuBlue™ NextGen is ideal for use in preparing samples for applications such as Next Generation Sequencing (NGS) and digital PCR.

The AccuBlue™ NextGen dsDNA quantitation assay is designed for use with fluorescence 96-well plate readers equipped with excitation and emission filters for detecting green fluorescence. The unique spectral properties of AccuBlue™ NextGen Dye make it especially well-suited for use with instruments with blue LED excitation sources. Because the AccuBlue™ NextGen assay is so sensitive, results will vary depending on many factors, such as microplate reader (see Table 1), assay volume, and pipet accuracy.

The AccuBlue™ NextGen dsDNA Quantitation Kit contains AccuBlue™ NextGen Dye, Enhancer, assay buffer, and dsDNA standard, with instructions for preparing a set of standards by serial dilution.

Kit components

  • AccuBlue™ NextGen Dye, 400X
  • AccuBlue™ NextGen Enhancer, 100X
  • AccuBlue™ NextGen Buffer, 1X
  • High Sensitivity dsDNA Standard *, 10 ng/uL

*Note: The concentration of the DNA Standard included in this kit (10 ng/uL) is different than the concentration of the DNA Standard (300 pg/uL) included in the previous version of this kit. The standard was changed due to DNA adsorption issues.


  • Linear range: 1-3000 pg dsDNA
  • Green fluorescence (Ex/Em 460/507 nm)
  • Unrivaled sensitivity (see Ref. 1)
  • Assay stability: prepared working solution can be stored for up to 6 months in the fridge for added convenience.
  • Green fluorescent dye is a perfect match for blue LED excitation sources
  • Green fluorescence detection compatible with fluorescence microplate readers, handheld fluorometers, and  NanoDrop® fluorospectrophotometers.
Table 1
readerassay format (assay volume)lowest DNA detected
BMG CLARIOStar96-well (200 uL)1 pg
BMG CLARIOStar384-well (50 uL)0.6 pg
Biotek Synergy H1m96-well (200 uL)5 pg
SpectraMax Gemini XS96-well (200 uL)5 pg
SpectraMax i3384-well (50 uL)1.25 pg

Other DNA Quantitation Kits:

dsDNA Quantitation KitLinear detection range (dsDNA in assay)Ex/Em (nm)Features
AccuClear™ Ultra High Sensitivity30 pg-250 ng468/507This kit offers great sensitivity and the widest linear range.

AccuBlue™ Broad Range2-2000 ng350/460For quantitation of high concentration DNA in a broad linear range. Assay can be extended to 4000 ng with minor loss of linearity.

AccuBlue™ NextGen1 pg*-3 ng468/507Quantitation of extremely precious or dilute samples. Optimal for sensitive applications such as NGS or digital PCR. *Limit of detection in the range of 1 pg to 5 pg depending on instrument sensitivity.
AccuBlue™ High Sensitivity0.2-100 ng485/530
AccuGreen™ High Sensitivity0.1-100 ng502/523Quantitation of DNA using the Qubit fluorometer from Thermo Fisher.

AccuBlue NextGen technology is covered by U.S. patents.

Reference 1: B. Bruijns, R. Tiggelaar, H. Gardeniers. Fluorescent cyanine dyes for the quantification of low amounts of dsDNA. Analytical Biochemistry (2016), doi: 10.1016/j.ab.2016.07.022.

Additional Information


Trial Size, 1,000 assays

Flyer (PDF): AccuBlue NextGen

Flyer (PDF): DNA Quantitation Kits

MSDS (PDF): msds-31060

Protocol (PDF): PI-31060

Shipment Method: Shipping and handling methods will be assessed and calculated at time of shipment based upon item(s) storage temperature conditions.

Expedited shipment may be requested at time of checkout.

Please note that products with recommended storage at 4°C or -20°C may ship at ambient temperature. This will not affect product performance. When you receive the product, place it under the recommended storage conditions.

More information about product handling and shelf life