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Content #1
Content #1
A blue nucleic acid gel stain to visualize dsDNA in agarose or polyacrylamide gels by the unaided eye.
Visualize DNA bands in gels by unaided eye and without UV light sources. Detection is highly sensitive and rivals most fluorescence-based gel stains.
DNAzure® Blue Nucleic Acid Gel Stain is a DNA-binding dye that turns from colorless to deep blue upon exposure to bright light. After color development, the stain also has broad emission near-infrared fluorescence that can be imaged using the LI-COR®, Odyssey®, or similar near-IR imaging systems. The sensitivity of detection is similar for visible color and near-IR imaging. DNAzure® is compatible with agarose gels or polyacrylamide gels as well as downstream applications such as sequencing and cloning. The dye efficiently removed from DNA by common gel extraction kits that utilize silica-based DNA purification columns.
Light exposure can be performed with a variety of white and blue light sources. For best results, we recommend performing the light exposure with the Glo-Plate™ White Photoactivation Device or Glo-Plate™ 2.0 Blue LED Illuminator.
We have tested a variety of different light sources for the development of DNAzure® bands in an agarose gel. We have found that most light sources will work, but how long the development takes is dependent on the brightness of the light. The fastest band development is seen with our Glo-Plate™ White Photoactivation Device and Glo-Plate™ 2.0 Blue LED Illuminator. We have also seen good results with other bright, white LED lights. Other light sources that work but take more time include an LED desk lamp and a cell phone light. We found that a 600W halogen lamp did not work well- it was too hot, which melted the gel.
We recommend storing DNAzure® at 4°C. After storage at room temperature, we have seen some loss of dye stability.
Yes, the 1X DNAzure® staining solution can be re-used for multiple gels, under certain conditions. Importantly, the staining solution must be removed from the gel before the gel is exposed to light to develop the bands. If the staining solution undergoes the light exposure, it will not be able to stain another gel. The used staining solution should be stored in the refrigerator, protected from light, between uses. We have successfully re-used the staining solution stored for up to 2 weeks, and used up to 4 times with little loss of signal (after 5 or 6 uses, the sensitivity was noticeably lower).