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Human IgG Heavy Chain Monoclonal Mouse Antibody (IG217 + IG266)

Product Attributes

Antibody number

#1262

Antibody type

Anti-Human Immunoglobulin, Primary

Host species

Mouse

Clonality

Monoclonal

Clone

IG217 + IG266

Isotype

IgG1, kappa + IgG2a, kappa

Molecular weight

75 kDa

Synonyms

G1m Marker; G2m Marker; G3m Marker; G4m Marker; HDC; Heavy Chain Disease Protein; Human Immunglobulin G; Ig gamma1/2/3/4 Chain C Region; IGHG1; IGHG2; IGHG3; IGHG4; Immunoglobulin Heavy Constant 1/2/3/4

Human gene symbol

IGHG

Entrez gene ID

3500; 3501; 3502; 3503

SwissProt

P01857, P01859, P01860, P01861

Unigene

510635

Immunogen

Purified human IgG

Verified antibody applications

IHC (FFPE) (verified)

Antibody target cellular localization

Secreted (extracellular)

Species reactivity

Human

Antibody application notes

Flow cytometry: 1 ug/million cells, Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody, Immunohistochemistry (formalin-fixed): 0.5-1 ug/mL for 30 minutes at RT, Immunofluorescence: 0.5-1 ug/mL, Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes, Optimal dilution for a specific application should be determined by user

Positive control

293T, Raji or hPBL cells. Tonsil or Spleen

Shipping condition

Room temperature

Storage Conditions

Store at 2 to 8 °C, Protect fluorescent conjugates from light, Note: store BSA-free antibodies at -10 to -35 °C

Regulatory status

For research use only (RUO)

Antibody/conjugate formulation

Conjugates: 0.1 mg/mL in PBS/0.1% BSA/0.05% azide, HRP conjugates: 0.1 mg/mL in PBS/0.05% BSA, Purified: 0.2 mg/mL in PBS/0.05% BSA/0.05% azide, Purified, BSA-free: 1 mg/mL in PBS without azide

Shelf life

Guaranteed for at least 24 months from date of receipt when stored as recommended

Cell/tissue expression

B-cells

Tumor expression

Leukemia/lymphoma

Antibody research areas

Cancer, Immunology, Inflammation

Conjugation
Don’t see what you’re looking for? Contact us for more conjugation options.
Note: Please be advised that primary antibodies with catalog numbers that begin with “BN” will only be offered conjugated to CF®405S, CF®488A, CF®568, CF®594, CF®640R, CF®647, and biotin as of 5/27/2022. However, previously available primary antibody conjugates that are in stock will remain available until inventory is exhausted. Please view the list of available conjugates on the product page. We also recommend our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with all CF® Dyes, fluorescent proteins, tandem dyes, and enzyme reporters. We apologize for the inconvenience.
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Product Description

This MAb is specific to heavy chain of IgG and shows minimal cross-reaction with heavy chains of other immunoglobulins. It is reactive with all subclasses of Gamma heavy chain(IgG1, IgG2a, IgG2b, IgG3). The most common feature of plasmacytomas, and certain non-Hodgkin’s lymphomas is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant. Ab-3 is superb for staining of formalin-fixed tissues.

 

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