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Human IgG Immunoglobulin Monoclonal Mouse Antibody (IG217)

Product Attributes

Antibody number

#0217

Antibody reactivity (target)

Human IgG

Antibody type

Anti-Human Immunoglobulin, Primary

Host species

Mouse

Clonality

Monoclonal

Clone

IG217

Isotype

IgG1, kappa

Molecular weight

75 kDa

Synonyms

G1m Marker; G2m Marker; G3m Marker; G4m Marker; HDC; Heavy Chain Disease Protein; Human Immunglobulin G; Ig gamma1/2/3/4 Chain C Region; IGHG1; IGHG2; IGHG3; IGHG4; Immunoglobulin Heavy Constant 1/2/3/4

Human gene symbol

IGHG

Entrez gene ID

3500 (IGHG1), 3501 (IGHG2), 3502 (IGHG3), 3503 (IGHG4)

SwissProt

P01857, P01859, P01860, P01861

Unigene

510635

Immunogen

Purified human Ig Gamma Chain

Verified antibody applications

IHC (FFPE) (verified)

Antibody target cellular localization

Cytoplasmic, Membrane/cell surface, Secreted (extracellular)

Species reactivity

Human

Antibody application notes

Immunohistology formalin/paraffin 0.5-1 ug/mL, Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes, Immunofluorescence 0.5-1 ug/mL, Flow Cytometry 0.5-1 ug/million cells/0.1 mL, Optimal dilution for a specific application should be determined by user

Positive control

293T, Raji or hPBL cells. Tonsil or Spleen

Shipping condition

Room temperature

Storage Conditions

Store at 2 to 8 °C, Protect fluorescent conjugates from light, Note: store BSA-free antibodies at -10 to -35 °C

Shelf life

Guaranteed for at least 24 months from date of receipt when stored as recommended

Regulatory status

For research use only (RUO)

Antibody/conjugate buffer formulation

Conjugates: 0.1 mg/mL in PBS/0.1% BSA/0.05% azide, HRP conjugates: 0.1 mg/mL in PBS/0.05% BSA, Purified: 0.2 mg/mL in PBS/0.05% BSA/0.05% azide, Purified, BSA-free: 1 mg/mL in PBS without azide

Cell/tissue expression

B-cells

Antibody research areas

Cancer, Immunology, Inflammation

Tumor expression

Leukemia/lymphoma

Conjugation
Don’t see what you’re looking for? Contact us for more conjugation options.
Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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Product Description

Recognizes a protein of 75 kDa, identified as γ heavy chain of human immunoglobulins. It reacts with all sub-classes of γ chain of human immunoglobulins. It does not cross-react with α (IgA), μ (IgM), ε (IgE), or δ (IgD), heavy chains, T-cells, monocytes, granulocytes, or erythrocytes. This MAb is useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin’s lymphomas. The most common feature of these malignancies is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant.

Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. See the CF® Dye Brochure for more information. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Stock status: Because Biotium offers a large number of antibody and conjugation options, primary antibody conjugates may be made to order. Typical lead times are up to one week for CF® dye and biotin conjugates, and up to 2-3 weeks for fluorescent protein and enzyme conjugates. Please email order@biotium.com to inquire about stock status and lead times before placing your order.

Catalog Number Key for Primary Antibody Conjugates

Antibody # prefixConjugationEx/Em (nm)Laser lineDetection channelSpecial applications/notes
BNC04CF®405S404/431405DAPI (microscopy), AF405SIM
BNC05CF®405M408/452405DAPI (microscopy), Pacific Blue™STORM, STED, SIM, 2-Photon, TIRF
BNC06CF®405L395/545405Pacific Orange™Spectral flow/imaging
BNC88CF®488A490/515488GFP, FITCSTORM, STED, 2-photon, TIRF
BNC14CF®514516/548488GFP, FITCSpectral flow/imaging
BNC43CF®543541/560532RFP, TRITC
BNC55CF®555555/565532, 561RFP, TRITCSTORM
BNC68CF®568562/583532, 561RFP, TRITCSTORM, STED, SIM, TIRF
BNC94CF®594593/614561Texas Red®STORM, STED, 2-photon
BNC40CF®640R642/662633-640Cy®5SIM, TIRF, and FLImP
BNC47CF®647650/665633-640Cy®5STORM
BNC60CF®660C667/685633-640Cy®5Spectral flow/imaging, STORM
BNC61CF®660R663/682633-640Cy®5Highly photostable
BNC80CF®680681/698633-640Cy®5.5, IR®700STORM, 3D SMLM, LI-COR® Odssey®
BNC81CF®680R680/701633-640Cy®5.5, IR®7002-photon, STED, SMT, LI-COR® Odyssey®; Highly photostable
BNC00CF®700695/720633-640AF700Spectral flow/imaging
BNC70CF®770770/797785, 808IR®800LI-COR® Odyssey®
BNCRR-PE (PE)496, 546,
565/578
488-561PE
BNCAAPC650/660594-633APC
BNCPPerCP482/677488-561PerCP, PE-Cy®5
BNCBBiotinN/AN/AN/A
BNCAPAlkaline
Phosphatase
N/AN/AN/A
BNCHHRPN/AN/AN/A
BNUBPurifiedN/AN/AN/A
BNUMPurified,
BSA-free
N/AN/AN/A
Alexa Fluor, Pacific Blue, Pacific Orange, and Texas Red are trademarks or registered trademarks of Thermo Fisher Scientific; Cy is a registered trademark of GE Healthcare; IRDye, LI-COR, and Odyssey are registered trademarks of LI-COR Bioscience.
 

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