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Mix-n-Stain™ Maxi HRP Antibody Labeling Kit, 1 mg Labeling

Simple and convenient antibody conjugation with HRP, with no purification required after labeling.

Product Attributes

Conjugation

HRP

Labeling scale

1 mg

Purification method

Purification not required after labeling

Size
Catalog #
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Qty
1 mg labeling
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Product Description

The Mix-n-Stain™ Maxi HRP Antibody Labeling Kit allows you to label 1 mg of IgG with horseradish peroxidase (HRP) in about 3 hours with minimal hands-on time. At the end of the reaction, the conjugate is ready for staining without further purification. Note: The labeling protocol and buffer requirements for Mix-n-Stain™ Maxi Kits differ from our original micro-scale Mix-n-Stain™ kits, see the Reaction Compatibility Chart below.

Features

  • Rapid, simple, covalent conjugation of antibody to HRP
  • Reaction takes about 3 hours, with minimal hands-on time
  • No purification required after labeling
  • One labeling reaction of 1 mg IgG per kit

Kit Components

  • HRP Reaction Buffer
  • Modified HRP
  • HRP Reaction Enhancer
  • Mix-n-Stain™ Quenching Buffer

Reaction Compatibility for Mix-n-Stain™ Maxi Antibody Labeling Kits, 1 mg Labeling

ComponentCompatibility
Sodium azideCompatible
Glycerol ≤ 10%: Compatible
Glycerol > 10%:Compatible after ultrafiltration*
Tris ≤20 mMCompatible
Tris >20 mMCompatible after ultrafiltration*
GlycineCompatible after ultrafiltration*
BSA or gelatinNot compatible; purify antibody**
Serum, cell culture supernatant, or ascites fluidNot compatible; purify antibody**
* Ultrafiltration vials are sold separately (catalog no. 22004).
** Protocol/reagents for antibody purification not provided.

Choose the right kit for your application

This kit is not designed to be used for multiple labeling reactions. For smaller-scale conjugation, see our original Mix-n-Stain™ HRP Antibody Labeling Kits.

Mix-n-Stain™ kits can be used to label other proteins, however, the ratio of HRP to protein may not be optimal for non-IgG proteins. Mix-n-Stain™ Kits are not recommended for labeling IgM isotype antibodies, because the labeling conditions may cause IgM to denature.

We offer a variety of kits for labeling antibodies and other proteins:

Learn about all of our Mix-n-Stain™ and protein labeling options, or see our Frequently Asked Questions (FAQs) for more information.

 

SNAP-tag and CLIP-tag are trademarks or registered trademarks of New England BioLabs; HaloTag is a registered trademark of Promega Corporation.
 

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