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PMA-Lite LED Photolysis Device

PMA-Lite™ LED Photolysis Device is specifically designed for photoactivation of PMAxx™, propidium monoazide (PMA) and other similar dyes.

PMA-Lite LED Photolysis Device
PMA-Lite LED Photolysis Device
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E90002

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Product Description

PMA-Lite™ LED Photolysis Device is specifically designed for photoactivation of propidium monoazide (PMA) and other similar dyes. It can also function as a general photolysis device to provide continuous illumination to 1.5-2 mL-sized vials in a controlled manner. The device can hold up to 18 vials. Multiple LED lights are positioned to provide even and maximal illumination from both the sides and bottom to all vials. An internal fan is used to reduce the heat generated from the lights to ensure near room temperature photolysis. The device has a timer that can be set for 10, 15, 20 or 30 minutes of continuous photolysis. The unit has 120/240V internal converter. A universal outlet adapter is included for customers outside of North America. The PMA-Lite™ LED Photolysis Device is covered by a warranty for two years from date of purchase. See page 2 of the Production Information Sheet for warranty details and instructions for obtaining service.

PMA (catalog nos. 40019 and 40013) is a photo-reactive dye that binds to dsDNA with high affinity and can be used for the selective detection of viable pathogens by qPCR.  PMAxx™ (catalog no. 40069) is a new and improved photoreactive dye for viability PCR. Learn more about PMA and PMAxx™.

Receive a free trial size of PMAxx™ with purchase of the PMA-Lite™ LED Photolysis Device.

PMA-Lite™ LED Photolysis Device. Each device comes with one removable blue tube adapter/rack.

Other PMA-related products:

For viability PCR of gram-negative bacteria we highly recommend using our new PMA Enhancer (31038) in conjunction with PMA. PMA Enhancer for Gram Negative Bacteria was designed to improve PMA-mediated discrimination between live and dead gram-negative bacteria. When a sequence from a gram-negative bacteria is amplified by PCR, samples pre-treated with Enhancer show a decrease in the signal from dead cells, with no change in the signal from live cells.

Biotium also provides strain-specific PMA Real-Time PCR Bacterial Viability kits with validated primers for 5 selected pathogens: M. tuberculosis, S. aureus, MRSA, Salmonella, and E. coli O157:H7. These kits provide everything that you need for the selective detection of your favorite species of live bacteria by real-time PCR. Don’t see your favorite strain? Let us know at techsupport@biotium.com.

Real-Time PCR Bacterial Viability Kits

References

1. Nocker, A., Cheung, C.Y., and Kamper, A.K. (2006). Comparison of propidium monoazide with ethidium monoazide for differentiation of live vs. dead bacteria by selective removal of DNA from dead cells. J. Microbio Meth. 67(2), 310-320.

2. Front. Microbiol. (2015), doi:10.3389/fmicb.2015.00295

3. Appl Microbiol Biotechnol (2015), doi: 10.1007/s00253-015-6715-x

Download the full PMA Reference List.

 

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Support & Faq

PMA and PMAxx™ for viability PCR

Biotium offers the PMA-Lite™ LED Photolysis Device for light-induced cross-linking of PMAxx™ and PMA to dsDNA. The PMA-Lite™ is designed for samples in microcentrifuge tubes.

For users that have samples in a different format (for example, 96-well plates or filters), we will soon be releasing a new light box that will allow for greater flexibility of assay format.

Commercial halogen lamps (>600 W) for home use have been employed for photoactivating PMA in some publications, though results have not been consistent due to inevitable variation in the set-up configurations. If you decide to use a halogen lamp, we recommend that you lay tubes on a block of ice set 20 cm from the light source, on a rocking platform to ensure continuous mixing. Set the lamp so that the light source is pointing directly downward onto the samples (up to 45° downward slant is OK). Expose samples to light for 5-15 min.

Category: PMA and PMAxx™ for viability PCR

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The LEDs in PMA-Lite have brightness of 600-800 millicandela (mcd). There are three LEDs next to each tube (one bottom, two side) and the wavelength is 465-475 nm.

Category: PMA and PMAxx™ for viability PCR

← FAQs

Both PMA and PMAxx™ are both offered as 20 mM solutions in water. We also offer PMA as a solid, and customers often ask whether it needs to be dissolved in DMSO. While in some publications users have prepared their PMA stock solution in DMSO, this is not necessary. Unlike the older viability dye EMA, which is not soluble in water, PMA and PMAxx™ are very water soluble.

Category: PMA and PMAxx™ for viability PCR

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Shipping/Shelf-life

Shipment Method: Shipping and handling methods will be assessed and calculated at time of shipment based upon item(s) storage temperature conditions. Expedited shipment may be requested at time of checkout. Please note that products with recommended storage at 4°C or -20°C may ship at ambient temperature. This will not affect product performance. When you receive the product, place it under the recommended storage conditions.