Support

Quick links
Frequently asked questions - categories
Ask a question
Contact Us
+1

PMA-Lite™ LED Photolysis Device

PMA-Lite™ LED Photolysis Device is specifically designed for photoactivation of PMAxx™, propidium monoazide (PMA) and other similar dyes.

Certificates of Analysis (COA)

Request COA
Size
Catalog #
price
Qty
Each
Clear selection
Add to wishlist
ADD TO CART
(Please select quantity above )

Wishlist updated! View wishlist

Shipping & Tax (CA only) not included in price. Learn more

Taxes: Tax is assessed in the state of California and is calculated during checkout based on your shipping information. Shipment Method: Shipping and handling methods will be assessed and calculated at time of shipment based upon item(s) storage temperature conditions. Please request expedited shipping in the Order Notes section if desired.

SPECIAL - Order 5 or more and receive 10% off.

Request a Bulk Quote

Product Description

The PMA-Lite™ LED Photolysis Device can be used to provide continuous illumination for up to 18 samples at a time, for convenient and controlled photoactivation of PMA, PMAxx™, and similar dyes.

Features:

  • Holds up to 18 vials (1.5-2 mL size)
  • Multiple LEDs provide even illumination to the sides and bottoms of all vials
  • Internal fan to maintain temperature ≤37°C
  • Timer for 10, 15, 20 or 30 minutes of photolysis
  • Universal outlet adapter included for customers outside North America
  • Two-year warranty from date of purchase

Specifications:

  • Dimensions (W x D x H): 8.25 x 6.25 x 2.625 in. (21 x 15.9 x 6.7 cm)
  • Weight: 3 lb. 10.7 oz. (1.66 kg)
  • Frequency Range: 50~60 Hz
  • Power Range: 100~240 VAC
  • Internal 120/240 V converter
  • Maximum Power: 60 W
  • LED Output Wavelength: 465-475 nm

PMA for viability PCR

PMA is a photo-reactive dye that binds to dsDNA with high affinity and can be used for the selective detection of viable pathogens by qPCR.  PMAxx™ is a new and improved photoreactive dye for viability PCR. Learn more about PMA and PMAxx™. Each purchase of PMA-Lite™ Photolysis Device includes a free trial size of PMAxx™.


Other PMA-related products:

We also offer the Glo-Plate™ Blue, a flat LED illuminator for viability PCR. While our standard PMA-Lite™ LED photolysis device was designed for use with microcentrifuge tubes, the Glo-Plate™ Blue device allows for more flexible assay set-ups, including multi-well plates for high-throughput assays. The base of the Glo-Plate™ Blue chamber contains blue LED lights topped with a diffuser, allowing for even illumination across samples.

For viability PCR of gram-negative bacteria we highly recommend using our new PMA Enhancer (31038) in conjunction with PMA. PMA Enhancer for Gram Negative Bacteria was designed to improve PMA-mediated discrimination between live and dead gram-negative bacteria. When a sequence from a gram-negative bacteria is amplified by PCR, samples pre-treated with Enhancer show a decrease in the signal from dead cells, with no change in the signal from live cells.

Also see our strain-specific PMA Real-Time PCR Bacterial Viability Kits with validated primers for 5 selected pathogens: M. tuberculosis, S. aureus, MRSA, Salmonella, and E. coli O157:H7. These kits provide everything that you need for the selective detection of your favorite species of live bacteria by real-time PCR.

References

1. Nocker, A., Cheung, C.Y., and Kamper, A.K. (2006). Comparison of propidium monoazide with ethidium monoazide for differentiation of live vs. dead bacteria by selective removal of DNA from dead cells. J. Microbio Meth. 67(2), 310-320.

2. Front. Microbiol. (2015), doi:10.3389/fmicb.2015.00295

3. Appl Microbiol Biotechnol (2015), doi: 10.1007/s00253-015-6715-x

Download the full PMA Reference List.

 

You may also like…

Support & Faq

PMA and PMAxx™ for viability PCR

Viability PCR originally used EMA (ethidium monoazide) to inactivate dead cell DNA. Biotium developed PMA (propidium monoazide) in collaboration with investigators at Montana State University (Nocker et al. 2006). PMA is more selective for dead cells than EMA, and became widely used for selective detection of viable microbes and viruses. PMAxx™ is Biotium’s newest viability PCR dye, designed to be more effective than PMA at eliminating PCR amplification of dead cell DNA. Therefore it provides the best discrimination between live and dead bacteria.

Learn more about PMA and PMAxx™ for viability PCR.

Category: PMA and PMAxx™ for viability PCR

← FAQs

Biotium offers the PMA-Lite™ LED Photolysis Device for light-induced cross-linking of PMAxx™ and PMA to dsDNA. The PMA-Lite™ is designed for samples in microcentrifuge tubes.

For users that have samples in a different format (for example, 96-well plates or filters), we will soon be releasing a new light box that will allow for greater flexibility of assay format.

Commercial halogen lamps (>600 W) for home use have been employed for photoactivating PMA in some publications, though results have not been consistent due to inevitable variation in the set-up configurations. If you decide to use a halogen lamp, we recommend that you lay tubes on a block of ice set 20 cm from the light source, on a rocking platform to ensure continuous mixing. Set the lamp so that the light source is pointing directly downward onto the samples (up to 45° downward slant is OK). Expose samples to light for 5-15 min.

Category: PMA and PMAxx™ for viability PCR

← FAQs

The LEDs in PMA-Lite have brightness of 600-800 millicandela (mcd). There are three LEDs next to each tube (one bottom, two side) and the wavelength is 465-475 nm.

Category: PMA and PMAxx™ for viability PCR

← FAQs

← FAQs

Ask a question

Shipping/Shelf-life

Shipment Method: Shipping and handling methods will be assessed and calculated at time of shipment based upon item(s) storage temperature conditions. Please request expedited shipping in the Order Notes section if desired. Please note that products with recommended storage at 4°C or -20°C may ship at ambient temperature. This will not affect product performance. When you receive the product, place it under the recommended storage conditions.