Support

Quick links
Frequently asked questions - categories
Ask a question
Contact Us
+1

Mix-n-Stain™ Fluorescent Protein & Tandem Dye Antibody Labeling Kits

Easily label your antibody with phycobiliproteins APC, R-PE and PerCP, or tandem dyes APC-CF®750T and RPE-CF®647T, in about 4 hours. The labeling procedure tolerates many common buffer components and antibody stabilizers.

Conjugation
Size
Catalog #
price
Qty
1 mg labeling
25-50 ug labeling
50-100 ug labeling
Clear selection
Add to wishlist
ADD TO CART
(Please select quantity above )

Wishlist updated! View wishlist

Shipping, Handling & Tax (CA only) not included in price. Learn more

Tax is assessed in the state of California. All fees will be calculated during checkout based on your shipping information and are subject to change.

SPECIAL - Order 5 or more and receive 10% off.

Request a Bulk Quote

Product Description

Mix-n-Stain™ Fluorescent Protein & Tandem Dye Antibody Labeling Kits utilize a revolutionary antibody labeling technology that allows you to easily label your antibody with APC, R-PE, PerCP or tandem dyes (phycobiliprotein/small dye conjugates) in about 4 hours. Phycobilicoprotein-conjugated antibodies are used mainly for flow and not microscopy because they have very poor photostability. However, they have been used for FLISA. The labeling procedure tolerates many common buffer components and antibody stabilizers (see the product protocol under downloads for details). Choose from kit sizes to label 25-50 ug, 50-100 ug or 1 mg of your antibody.

Fluorescent proteins (APC, R-PE and PerCP):

  • APC (Allophycocyanin) is a bright phycobiliprotein of ~105 kDa, found in red algae and cyanobacteria (blue-green algae). It has absorption maxima at 650 and emission at 660 nm. Because of the high fluorescence quantum yield (~0.68), APC is by far the brightest dye for the 650 nm wavelength region.
  • R-PE (R-phycoerythrin, also called PE) is a large phycobiliprotein with a molecular weight of ~240 kDa. It has absorption maxima at 496, 546, and 565 nm respectively and emission at 578 nm. Because of the high extinction coefficient (~1,960,000) and high fluorescence quantum yield (~0.82), R-PE is by far the brightest dye for the 565 nm wavelength region. R-PE is one of the brightest fluorochromes for flow cytometry, but due to its photobleaching properties it is not recommended for fluorescence microscopy.
  • PerCP (Peridinin Chlorophyll Protein Complex) is a small, 35 kDa, phycobiliprotein found in dinoflagellates. PerCP has absorption maxima at 482 nm and emission at 677 nm.

Tandem dyes:

Tandem dyes are composed of two covalently attached fluorescent molecules. An example of this a phycobiliprotein to which small molecule dyes are conjugated. By a process called Förster resonance energy transfer (FRET), or fluorescence resonance energy transfer, the tandem dye has the excitation properties of the donor molecule and the emission properties of the acceptor. The Stokes shift of the tandem dye is larger than that of the original donor molecule. Tandem dyes are used almost exclusively for flow cytometry because of imperfect energy transfer, and because they show bleed-through fluorescence in the acceptor dye channel, which requires compensation. Aside from cytometers, most fluorescence instruments don’t perform compensation and are generally not configured for the long-stokes shifts of tandem dyes.

  • The Mix-n-Stain™ RPE-CF®647T Antibody Labeling Kit is for labeling an antibody with tandem RPE-CF®647T (Ex/Em 496, 546, 555/665 nm) for multi-color flow cytometry. It is designed for detection in the RPE-Cy®5 channel.
  • The Mix-n-Stain™ APC-CF®750T Antibody Labeling Kit is for labeling an antibody with tandem APC-CF®750T (Ex/Em 650/780 nm) for multi-color flow cytometry. It is designed for detection in the APC-Cy®7 channel.

Mix-n-Stain™ Fluorescent Protein Antibody Labeling Kits

Product descriptionExcitation/emissionCatalog numberReaction size Product protocolLabeling time
Mix-n-Stain™ R-PE (PE) Antibody Labeling Kit496, 546, 565/
578 nm
9229825-50 ug antibody
PI-Mix-n-Stain™ R-PE
~4 hours (~15 minutes hands-on time)
9229950-100 ug antibody
Mix-n-Stain™ APC Antibody Labeling Kit650/660 nm9230625-50 ug antibody
PI-Mix-n-Stain™ APC
9230750-100 ug antibody
Mix-n-Stain™ PerCP Antibody Labeling Kit482/677 nm9230825-50 ug antibody
PI-Mix-n-Stain™ PerCP
9230950-100 ug antibody
Mix-n-Stain™ RPE-CF®647T Antibody Labeling Kit496/665
(PE-Cy®5 channel)
9234025-50 ug antibody
PI-Mix-n-Stain™ RPE-CF®647
~2 hours (few minutes hands-on time)
9234150-100 ug antibody
923461 mg antibody
Mix-n-Stain™ APC-CF®750T Antibody Labeling Kit650/780 nm
(APC-Cy®7 channel)
9231025-50 ug antibody
PI-Mix-n-Stain™ APC-CF®750T
9231150-100 ug antibody
Cy Dye is a registered trademark of GE Healthcare.

References

Download a list of Mix-n-Stain™ references.

 

You may also like…

    Mix-n-Stain™ CF® Dye Antibody Labeling Kits

    Mix-n-Stain™ antibody labeling kits are a revolutionary antibody labeling technology that allows you to label your antibody with biotin or one of our superior CF® dyes in 30 minutes without a purification step. The labeling procedure tolerates many common buffer components and antibody stabilizers. Now with an extended shelf life of at least 1 year.

    Mix-n-Stain™ Biotin Antibody Labeling Kit

    Mix-n-Stain™ antibody labeling kits are a revolutionary antibody labeling technology that allows you to label your antibody with biotin or one of our superior CF® dyes in 30 minutes without a purification step. The labeling procedure tolerates many common buffer components and antibody stabilizers. Now with an extended shelf life of at least 1 year.

    Mix-n-Stain™ FITC Antibody Labeling Kit

    Mix-n-Stain™ antibody labeling kits are a revolutionary antibody labeling technology that allows you to label your antibody with biotin or one of our superior CF® dyes in 30 minutes without a purification step. The labeling procedure tolerates many common buffer components and antibody stabilizers.

    Live-or-Dye™ Fixable Viability Staining Kits

    Live-or-Dye™ Fixable Viability Staining Kits are designed for discrimination between live and dead cells during flow cytometry. Live/dead stains are useful probes to include when analyzing cell surface protein expression by flow cytometry, because they allow intracellular fluorescence signal from dead cells with permeable plasma membranes to be excluded from analysis.

    Flow Cytometry Fixation/Permeabilization Kit

    Flow Cytometry Fixation/Permeabilization Kit contains optimally formulated buffers for fixation and permeabilization of suspension cells for immunofluorescence staining of intracellular antigens for analysis by flow cytometry. Note: the fixation buffer in this kit is prepared from methanol-stabilized formaldehyde. For methanol-free fixative, see our Paraformaldehyde, 4% in PBS, Ready-to-Use Fixative.

    10X Phosphate-Buffered Saline (PBS)

    Molecular biology grade 10X phosphate-buffered saline (PBS). RNase-, DNase-, protease-free.