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Mix-n-Stain™ Fluorescent Protein & Tandem Dye Antibody Labeling Kits

Easily label your antibody with phycobiliproteins APC, R-PE (PE), PerCP, or tandem dyes in about 4 hours with no purification step.

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50-100 ug labeling
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Product Description

Mix-n-Stain™ Fluorescent Protein & Tandem Dye Antibody Labeling Kits allow you to label your antibody with a fluorescent phycobiliprotein in about 4 hours with no purification. The labeling procedure tolerates many common buffer components and antibody stabilizers (see product protocols for details).

  • Choice of APC, R-PE (PE), PerCP, or tandem dyes
  • Labeling takes a few hours with minimal hands-on time
  • Kit sizes for labeling 25-50 ug, 50-100 ug or 1 mg IgG
  • No purification, 100% recovery

Fluorescent proteins (APC, R-PE and PerCP):

  • APC (Allophycocyanin) is a bright phycobiliprotein of ~105 kDa, found in red algae and cyanobacteria (blue-green algae). It has absorption maxima at 650 and emission at 660 nm. Because of the high fluorescence quantum yield (~0.68), APC is by far the brightest dye for the 650 nm wavelength region.
  • R-PE (R-phycoerythrin, also called PE) is a large phycobiliprotein with a molecular weight of ~240 kDa. It can be excited by 488, 532, and 561 nm laser lines and has an emission maxima at 576 nm. Because of the high extinction coefficient (~1,960,000) and high fluorescence quantum yield (~0.82), R-PE is by far the brightest dye for the 565 nm wavelength region. R-PE is one of the brightest fluorochromes for flow cytometry, but due to its photobleaching properties it is not recommended for fluorescence microscopy.
  • PerCP (Peridinin Chlorophyll Protein Complex) is a small, 35 kDa, phycobiliprotein found in dinoflagellates. PerCP has absorption maxima at 482 nm and emission at 677 nm.

Tandem dyes:

Tandem dyes are composed of two covalently attached fluorescent molecules. An example of this a phycobiliprotein to which small molecule dyes are conjugated. By a process called Förster resonance energy transfer (FRET), or fluorescence resonance energy transfer, the tandem dye has the excitation properties of the donor molecule and the emission properties of the acceptor. The Stokes shift of the tandem dye is larger than that of the original donor molecule. Tandem dyes are used almost exclusively for flow cytometry because of imperfect energy transfer, and because they show bleed-through fluorescence in the acceptor dye channel, which requires compensation. Aside from cytometers, most fluorescence instruments don’t perform compensation and are generally not configured for the long-stokes shifts of tandem dyes.

We offer kits for labeling with the following tandem dyes featuring Biotium’s next-generation CF® Dyes with superior brightness.

  • RPE-CF®583R (Ex/Em 488, 532, 561/608 nm) for the RPE-Texas Red® channel
  • RPE-CF®647T (Ex/Em 488, 532, 561/673 nm) for the RPE-Cy®5 channel
  • APC-CF®750T (Ex/Em 633, 640/785 nm) for the APC-Cy®7 channel
  • APC-CF®790T (Ex/Em 633, 640/814 nm) for the APC-Cy®7 channel

Note: APC-CF®790T is intended for spectral flow cytometry. For conventional flow cytometry use APC-CF®750T.

We also offer Mix-n-Stain™ Kits for labeling antibodies, nanobodies, or small ligands with bright & photostable CF® Dyes and other labels. See our full line of Antibody & Protein Labeling Kits.

Mix-n-Stain™ Fluorescent Protein Antibody Labeling Kits

Mix-n-Stain™ R-PE
Antibody Labeling Kit
496, 546, 565/
578 nm
92298, 9245225-50 ug IgG
PI-Mix-n-Stain™ R-PE
~4 hours
(~15 min. hands-on)
9229950-100 ug IgG
Mix-n-Stain™ APC
Antibody Labeling Kit
650/660 nm92306, 9245325-50 ug IgG
PI-Mix-n-Stain™ APC
9230750-100 ug IgG
Mix-n-Stain™ PerCP
Antibody Labeling Kit
482/677 nm9230825-50 ug IgG
PI-Mix-n-Stain™ PerCP
9230950-100 ug IgG
Mix-n-Stain™ RPE-CF®583R
Antibody Labeling Kit
488, 532, 561/608 nm
(PE-Texas Red®5 channel)
9244225-50 ug IgG
PI-Mix-n-Stain™ RPE-CF® Dye
~2 hours
(few min. hands-on)
9244350-100 ug IgG
Mix-n-Stain™ RPE-CF®647T
Antibody Labeling Kit
488, 532, 561/673 nm
(PE-Cy®5 channel)
9234025-50 ug IgG
PI-Mix-n-Stain™ RPE-CF® Dye
9234150-100 ug IgG
923461 mg IgG
Mix-n-Stain™ APC-CF®750T
Antibody Labeling Kit
633, 640/785 nm
(APC-Cy®7 channel)
9231025-50 ug IgG
PI-Mix-n-Stain™ APC-CF® Dye
9231150-100 ug IgG
Mix-n-Stain™ APC-CF®790T
Antibody Labeling Kit
633, 640/814 nm
(APC-Cy®7 channel)
9234725-50 ug IgG
9234850-100 ug IgG
Note: Kits are not recommended for labeling IgM antibodies. Cy Dye is a registered trademark of Cytiva.


Download a list of Mix-n-Stain™ references.


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