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Mix-n-Stain™ Fluorescent Protein & Tandem Dye Antibody Labeling Kits

Easily label your antibody with phycobiliproteins APC, R-PE (PE), PerCP, or tandem dyes in about 4 hours with no purification step.

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Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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25-50 ug labeling
50-100 ug labeling
1 mg labeling
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Product Description

Mix-n-Stain™ Fluorescent Protein & Tandem Dye Antibody Labeling Kits allow you to label your antibody with a fluorescent phycobiliprotein in about 4 hours with no purification. The labeling procedure tolerates many common buffer components and antibody stabilizers (see product protocols for details).

  • Choice of APC, R-PE (PE), PerCP, or tandem dyes
  • Labeling takes a few hours with minimal hands-on time
  • Kit sizes for labeling 25-50 ug, 50-100 ug or 1 mg IgG
  • No purification, 100% recovery

Fluorescent proteins (APC, R-PE and PerCP):

  • APC (Allophycocyanin) is a bright phycobiliprotein of ~105 kDa, found in red algae and cyanobacteria (blue-green algae). It has absorption maxima at 650 and emission at 660 nm. Because of the high fluorescence quantum yield (~0.68), APC is by far the brightest dye for the 650 nm wavelength region.
  • R-PE (R-phycoerythrin, also called PE) is a large phycobiliprotein with a molecular weight of ~240 kDa. It has absorption maxima at 496, 546, and 565 nm respectively and emission at 578 nm. Because of the high extinction coefficient (~1,960,000) and high fluorescence quantum yield (~0.82), R-PE is by far the brightest dye for the 565 nm wavelength region. R-PE is one of the brightest fluorochromes for flow cytometry, but due to its photobleaching properties it is not recommended for fluorescence microscopy.
  • PerCP (Peridinin Chlorophyll Protein Complex) is a small, 35 kDa, phycobiliprotein found in dinoflagellates. PerCP has absorption maxima at 482 nm and emission at 677 nm.

Tandem dyes:

Tandem dyes are composed of two covalently attached fluorescent molecules. An example of this a phycobiliprotein to which small molecule dyes are conjugated. By a process called Förster resonance energy transfer (FRET), or fluorescence resonance energy transfer, the tandem dye has the excitation properties of the donor molecule and the emission properties of the acceptor. The Stokes shift of the tandem dye is larger than that of the original donor molecule. Tandem dyes are used almost exclusively for flow cytometry because of imperfect energy transfer, and because they show bleed-through fluorescence in the acceptor dye channel, which requires compensation. Aside from cytometers, most fluorescence instruments don’t perform compensation and are generally not configured for the long-stokes shifts of tandem dyes.

We offer kits for labeling with the following tandem dyes featuring Biotium’s next-generation CF® Dyes with superior brightness.

  • RPE-CF®647T (Ex/Em 496, 546, 555/665 nm) for the RPE-Cy®5 channel
  • APC-CF®750T (Ex/Em 650/780 nm) for the APC-Cy®7 channel

We also offer Mix-n-Stain™ Kits for labeling antibodies, nanobodies, or small ligands with bright & photostable CF® dyes and other labels. See our full line of Antibody & Protein Labeling Kits.

Mix-n-Stain™ Fluorescent Protein Antibody Labeling Kits

Product
Name
Ex/EmCatalog
Number
Reaction
Size
Labeling
Time
Protocol
Mix-n-Stain™ R-PE
Antibody Labeling Kit
496, 546, 565/
578 nm
9229825-50 ug IgG~4 hours
(~15 min. hands-on)

PI-Mix-n-Stain™ R-PE
9229950-100 ug IgG
Mix-n-Stain™ APC
Antibody Labeling Kit
650/660 nm9230625-50 ug IgG
PI-Mix-n-Stain™ APC
9230750-100 ug IgG
Mix-n-Stain™ PerCP
Antibody Labeling Kit
482/677 nm9230825-50 ug IgG
PI-Mix-n-Stain™ PerCP
9230950-100 ug IgG
Mix-n-Stain™ RPE-CF®647T
Antibody Labeling Kit
496/665
(PE-Cy®5 channel)
9234025-50 ug IgG~2 hours
(few min. hands-on)

PI-Mix-n-Stain™ RPE-CF®647
9234150-100 ug IgG
923461 mg IgG
Mix-n-Stain™ APC-CF®750T
Antibody Labeling Kit
650/780 nm
(APC-Cy®7 channel)
9231025-50 ug IgG
PI-Mix-n-Stain™ APC-CF®750T
9231150-100 ug IgG
Note: Kits are not recommended for labeling IgM antibodies. Cy Dye is a registered trademark of GE Healthcare.

References

Download a list of Mix-n-Stain™ references.

 

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