Cell & Organelle Stains

Far-red, mitochondrial stain with excellent tolerance for fixation and permeabilization after staining.

Fluorogenic mitochondrial stains for live cells that rapidly accumulate in mitochondria and can be imaged without washing.

Oxazole Blue, also known as PO-PRO™-1, is a blue-fluorescent, cell-impermeant nucleic acid stain that can be used to stain dead or fixed cells.

A highly selective actin filament stain for fixed and permeabilized cells. Labeled phalloidins have similar affinity for both large and small filaments.

PHA-L triggers lymphocyte agglutination and is available conjugated to biotin and a selection of 6 bright and photostable CF® Dyes.

SNA lectin binds to sialic acid attached to terminal galactose. SNA lectin is a carbohydrate-binding protein that can induce apoptosis in insect cells and cultured mammalian cell cancer cells. Biotium offers SNA lectin conjugated to biotin and a selection of 6 bright and photostable CF® Dyes.

UEA I binds to glycoproteins and glycolipids containing α-linked fucose residues, such as ABO blood group glycoconjugates. The lectin is an established marker for human endothelial cells and has been used to differentiate between angiosarcomas and epithelial tumors. Biotium offers UEA I conjugated to biotin and a selection of 6 bright and photostable CF® Dyes.

A wheat germ agglutinin (WGA) lectin labeled with Biotium’s CF® Dyes, biotin, or HRP. It is a carbohydrate-binding lectin that has high affinity for sialic acid and N-acetylglucosamine and stains yeast bud scars as well as the cell membrane of gram+ bacteria and mammalian cells.

A high affinity inhibitor of the nicotinic acetylcholine receptor in the neuromuscular junction. Blocks acetylcholine activity at the postsynaptic membrane.

1,6-Diphenyl-1,3,5-hexatriene (DPH) is almost non-fluorescent in water, but it exhibits a strong increase in fluorescence after intercalation into membranes and can be used as a probe for viscosity, polarity and lipid order.

4-Di-1-ASP is a styryl dye that stains mitochondria of live cells. It has also been used to stain glioma cells in living brain tissues.

5(6)-CFDA (full name: 5-(and-6)-Carboxyfluorescein diacetate) is membrane-permeant and thus can be loaded into cells via incubation. Once inside cells, CFDA is hydrolyzed by intracellular esterases to carboxyfluorescein (see #51013 ).

5-(and-6)-carboxy-2′,7′-dichlorofluorescein is similar to carboxyfluorescein (51013), but has lower pK_a. It is useful for detecting pH in more acidic environment.

5-(and-6)-FAM (full name: 5-(and-6)-Carboxyfluorescein mixed isomer) has a pKa of 6.5 and can be used as a pH indicator.

This dye can be used as a low pH indicator similar to 51015, and is also useful as a general green fluorescent label.

The pKa of 5-carboxy-2′,7′-dichlorosulfonefluorescein is near 4 and thus the dye is potentially an excellent pH indicator for acidic organelles.