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Nonyl Acridine Orange (NAO)

NAO is a green fluorescent mitochondrial dye whose staining is not dependent on mitochondrial membrane potential.

Product Attributes

CAS number

75168-11-5

Cellular localization

Mitochondria

For live or fixed cells

For live/intact cells

Assay type/options

No-wash staining, Real-time imaging

Potential dependence

Mitochondrial potential-independent

Colors

Red

Excitation/Emission

495/522 nm

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Product Description

NAO is a green fluorescent mitochondrial dye whose staining is not dependent on mitochondrial membrane potential (1), unlike JC-1 (70011) or Rhodamine 123 (70010). NAO has been used to study multidrug resistance (2) and to measure changes in mitochondrial mass during apoptosis (3). The dye is reported to bind cardiolipin, a phosphoprotein in the inner mitochondrial membrane (4). Also see our MitoView™ Dyes and other mitochondrial dyes.

  • λExEm (MeOH) = 495/522 nm
  • ε (MeOH) = 63,000
  • Orange solid soluble in DMSO or DMF
  • Store at 4°C and protect from light, especially in solution
  • C26H38BrN3
  • MW: 473
  • [75168-11-5]

References

1. Cytometry 9, 206 (1988).

2. Cancer Res 51, 4665 (1991).

3. Exp Cell Res 214, 323 (1994).

4. FEBS Letters 260, 236 (1990).

 

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