Fluorescent Proteins, Nucleotides & Other Conjugates

Hydroxystilbamidine (also called Fluoro-Gold™) has been used extensively as a retrograde tracer for neurons and also a histochemical stain. The 4% solution in deionized H₂O is a convenient, ready-made solution.

RuBP-2S (Ruthenium(bis(bathophenanthroline))(bathophenanthrolinedisulfonate) is a luminescent ruthenium complex similar to RuBPS (80021).

RuBPS (Ruthenium(tris(bathophenanthrolinedisulfonate), tetrasodium salt) is a negatively-charged water-soluble luminescent ruthenium complex. The complex can be efficiently excited by either UV light or visible light in the wavelength range from 400 nm to 500 nm with bright red emission centered around 605 nm.

We developed biotin-rhodamine 110 as an alternative to biotin-4-fluorescein (90062) and fluorescein biotin (80019), both of which have been used for detection of biotin binding sites and the degree of biotinylation of proteins, and for the measurement of avidin and streptavidin in crude biological samples.

Like Lucifer Yellow CH, Lucifer Yellow Cadaverine can be used as a fixable fluorescent tracer. In addition, the dye can be used to conjugate to carboxylic acid groups.

Fluorescein biotin (5-((N-(5-(N-(6-(Biotinoyl)amino)hexanoyl)amino)pentyl)thioureidyl) fluorescein) can be used to detect and quantify biotin binding sites and the degree of biotinylation of proteins. Also see biotin-rhodamine 110 (80022) and biotin-4-fluorescein (90062).

This fluorescent biotin derivative is a useful tracer for studying neuronal morphology. The biotin portion of the molecule allows researchers to amplify the fluorescent signal using standard biotin-avidin or biotin-streptavidin biochemistry.

Lucifer Yellow CH lithium salt (LY CH lithium salt) is a widely used polar molecular tracer for studying neuronal morphology. The fluorescent molecule contains a carbohydrazide that allow the molecule to be aldehyde-fixable.

Lucifer Yellow CH potassium salt (LY CH potassium salt) is a widely used polar molecular tracer for studying neuronal morphology. The fluorescent molecule contains a carbohydrazide that allow the molecule to be aldehyde-fixable.

Calcein is a highly negatively charged, water-soluble fluorescein derivative. Its fluorescence is nearly independent of pH in the range between 6.5 and 12.

Hydroxystilbamidine (also called Fluoro-Gold™) has been used extensively as a retrograde tracer for neurons and also a histochemical stain.

DPX is a positively charged quencher that is often used with ANTS (90010) to study membrane fusion or permeability.

Biotin-AM3-25 is a conjugate of AM3-25 and biotin. Membranes labeled with the probe can subsequently couple to avidin or streptavidin.

The pKa of 5-carboxy-2′,7′-dichlorosulfonefluorescein is near 4 and thus the dye is potentially an excellent pH indicator for acidic organelles.

6-CFDA is membrane-permeant and thus can be loaded into cells via incubation. Once inside cells, 6-CFDA is hydrolyzed by intracellular esterases to 6-carboxyfluorescein.

5-CFDA is membrane-permeant and thus can be loaded into cells via incubation. Once inside cells, 5-CFDA is hydrolyzed by intracellular esterases to 5-carboxyfluorescein (see 50015).