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5-CFDA (5-Carboxyfluorescein diacetate, single isomer)

5-CFDA is membrane-permeant and thus can be loaded into cells via incubation. Once inside cells, 5-CFDA is hydrolyzed by intracellular esterases to 5-carboxyfluorescein (see 50015).

Product Attributes

CAS number

79955-27-4

Cell permeability

Membrane permeant

Probe cellular localization

Nucleus & cytoplasm

pKa

pKa 6.5

Indicator type

Ratiometric

Colors

Green

Excitation/Emission

495/519 nm (pH 9) (after hydrolysis)

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Qty
100 mg
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Protocols
SDS

Product Description

5-CFDA is membrane-permeant and thus can be loaded into cells via incubation. Once inside cells, 5-CFDA is hydrolyzed by intracellular esterases to 5-carboxyfluorescein (see 50015).

  • Off-white solid soluble in DMSO
  • Store desiccated at -20°C
  • C25H16O9
  • MW: 460
  • [79955-27-4]

References

1. J Cell Sci 98, 343(1991).

2. Nature 295, 524 (1982).

3. J Immunol Meth 100, 261 (1987).

4. J Immunol Meth 33, 33 (1980).

 

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