Fluorescent Proteins, Nucleotides & Other Conjugates

This dye can be used as a low pH indicator similar to 51015, and is also useful as a general green fluorescent label.

5-(and-6)-carboxy-2′,7′-dichlorofluorescein is similar to carboxyfluorescein (51013), but has lower pK_a. It is useful for detecting pH in more acidic environment.

5-(and-6)-Carboxy-2′,7′-dichlorofluorescein diacetate is membrane-permeant and thus can be loaded into cells via incubation. The diacetate is readily hydrolyzed to 5-(and-6)-carboxy-2′,7′-dichlorofluorescein (51015) by intracellular esterases.

5-(and-6)-FAM (full name: 5-(and-6)-Carboxyfluorescein mixed isomer) has a pKa of 6.5 and can be used as a pH indicator.

Biotin-11-dCTP (Biotin-11-2′-deoxycytidine-5′-triphosphate, tetralithium salt) can be enzymatically incorporated into DNA via nick translation, random priming, or 3′-end terminal labeling. The number 11 is the number of atoms in the linker between biotin and dCTP.

This product is similar to biotin-11-UTP (40033) and biotin-16-UTP (40023) except that it has a longer and water soluble spacer group, which should facilitate interaction between the biotin group and avidin or streptavidin.

Biotin-11-CTP (Biotin-11-cytidine-5′-triphosphate, tetralithium salt) can be enzymatically incorporated into RNA.

Biotin-11-UTP can be enzymatically incorporated into RNA. The number 11 is the number of atoms in the linker between biotin and UTP.

This product is similar to biotin-11-dUTP (40029) and biotin-16-dUTP (40022) except that it has a longer and water-soluble spacer group, which should facilitate interaction between the biotin group and avidin or streptavidin.

This product is similar to biotin-11-dUTP (40029) and biotin-16-dUTP (40022) except that it has a longer and water-soluble spacer group, which should facilitate interaction between the biotin group and avidin or streptavidin.

Biotin-11-dUTP can be enzymatically incorporated into DNA via nick translation, random priming, or 3′-end terminal labeling. The number ’11’ is the number of atoms in the linker between biotin and dUTP.

Biotin-11-dUTP can be enzymatically incorporated into DNA via nick translation, random priming, or 3′-end terminal labeling. The number ’11’ is the number of atoms in the linker between biotin and dUTP.

Biotin-16-dUTP can be enzymatically incorporated into DNA via nick translation, random priming, or 3′ end terminal labeling. The terminal deoxynucleotidyl transferase (TdT)-mediated biotin-dUTP nick end-labeling (TUNEL) method has been commonly used for apoptosis studies.

Biotin-16-UTP can be enzymatically incorporated into RNA. The number 16 indicates the number of atoms between biotin and UTP.

Biotin-16-dUTP can be enzymatically incorporated into DNA via nick translation, random priming, or 3′ end terminal labeling. The terminal deoxynucleotidyl transferase (TdT)-mediated biotin-dUTP nick end-labeling (TUNEL) method has been commonly used for apoptosis studies.

5-Aminoallyl-dUTP can be enzymatically incorporated into DNA. The resulting amine-containing DNA can be subsequently labeled with a fluorescent dye, biotin or other haptens via conventional peptide coupling method.