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5-(and-6)-Carboxyfluorescein

5-(and-6)-FAM (full name: 5-(and-6)-Carboxyfluorescein mixed isomer) has a pKa of 6.5 and can be used as a pH indicator.

Product Attributes

CAS number

72088-94-9

Cell permeability

Membrane impermeant

Probe cellular localization

Fluid phase tracer

pKa

pKa 6.5

Indicator type

Ratiometric

Colors

Green

Excitation/Emission

492/514 nm

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100 mg
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Product Description

5-(and-6)-FAM (full name: 5-(and-6)-Carboxyfluorescein mixed isomer) has a pKa of 6.5 and can be used as a pH indicator. Its excitation spectrum and fluorescence response to pH are similar to those of BCECF. Carboxyfluorescein can also be used as a tracer dye. The dye is membrane-impermeant and can be loaded into cells by microinjection or scrape loading.

  • λExEm (pH 9) = 492/514 nm
  • ε (pH 9) = 82,000
  • Yellow orange solid soluble in pH>6 water
  • Store at 4°C and protect from light, especially in solution
  • C21H12O7
  • MW: 376
  • [72088-94-9]

References

1. Meth Enzymol 128, 65 (1986).

 

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