Fluorescent Proteins, Nucleotides & Other Conjugates
BrdU (5-Bromo-2′-deoxyuridine)
BrdU (5-Bromo-2′-deoxyuridine) can be incorporated into DNA during cell division and subsequently detected by a BrdU antibody. The probe can be used to study cell-cycle kinetics (1).
BrUTP, 10 mM Solution
BrUTP (5-Bromouridine-5′-triphosphate) can be enzymatically incorporated into RNA and detected with anti-BrdU antibodies.
Calcein (High Purity)
Calcein is a highly negatively charged, water-soluble fluorescein derivative. Its fluorescence is nearly independent of pH in the range between 6.5 and 12.
Calcein AM
80011, 80011-1, 80011-2, - 80011-3View allHide
Calcein AM is a widely used green fluorescent cell marker. Calcein AM is membrane-permeant and thus can be introduced into cells via incubation.
CF® Dye Biocytin
92004, 92005, 92006, - 92007View allHide
CF® Dye biocytin is a cell-impermeant, fixable polar tracer that is a conjugate of CF® Dye and biotin with an aldehyde-fixable primary amine. Polar tracers are commonly used to investigate cell-cell and cell-liposome fusion as well as membrane permeability and transport through gap junctions or cell uptake during pinocytosis.
Cyanine 644-cAMP
00022, - 00022-1View allHide
A fluorescently labeled cAMP analog that can be used to probe cAMP receptors.
dCTP CF® Dye Conjugates
40027-T, 40028-T, 40055-T, 40056-T, 40057-T, 40058-T, 40066-T, 40067-T, 40068-T, 40027, 40028, 40055, 40056, 40057, 40058, 40066, 40067, - 40068View allHide
CF® dye dCTP can be used to synthesize labeled DNA probes for in-situ hybridization, microarray or blotting applications.
DEAC-dUTP
DEAC-dUTP (diethylaminocoumarin-dUTP) can be used to synthesize fluorescent DNA probes for in situ hybridization, microarray or blotting techniques. DEAC has absorption at 426 nm and emission at 480 nm.
Digoxigenin-dUTP, Alkali Stable
Digoxigenin-dUTP can be enzymatically incorporated into DNA via nick translation, random priming, or 3′-end terminal labeling to synthesize labeled DNA probes for in-situ hybridization, microarray or blotting techniques. The digoxigenin labeled probe could be detected by using fluorescent labeled or enzyme labeled anti-digoxigenin antibody.
DPA/Terbium for Membrane Fusion Assay
The principle of DPA/Tb3 for vesicle fusion assay is based on the fact that contact of the chelator dipicolinic acid (DPA) with terbium (III) forms an instant Tb3 -DPA complex that is ~10,000 times more fluorescent than free Tb3 . In the assay, separate vesicle populations are loaded with 2.5 mM TbCl3 in 50 mM sodium citrate, or 50 mM DPA in 20 mM NaCl.
DPX
DPX is a positively charged quencher that is often used with ANTS (90010) to study membrane fusion or permeability.
Fluorescein biotin
Fluorescein-12-dUTP
Fluorescein-12-dUTP can be used to synthesize fluorescent DNA probes.
Fluorescein-DHPE
Fluorescein-DHPE is a useful surface pH indicator, and has also been used for measuring lateral diffusion in membranes.
Hydroxystilbamidine (Fluoro-Gold™)
Hydroxystilbamidine (also called Fluoro-Gold™) has been used extensively as a retrograde tracer for neurons and also a histochemical stain.
Hydroxystilbamidine (Fluoro-Gold™), 4% in H2O
Hydroxystilbamidine (also called Fluoro-Gold™) has been used extensively as a retrograde tracer for neurons and also a histochemical stain. The 4% solution in deionized H2O is a convenient, ready-made solution.