Fluorescent Proteins, Nucleotides & Other Conjugates

5-TAMRA-dUTP (5-Tetramethylrhodamine-dUTP) can be enzymatically incorporated into DNA. Note: 5-TAMRA-dUTP may not be compatible with PCR labeling of DNA probes.

A biotinylated cyclic nucleotide that is an important second messenger in many biological processes and has been used as a tracer in immunoassays.

A fluorescently labeled cAMP analog that can be used to probe cAMP receptors.

A high affinity inhibitor of the nicotinic acetylcholine receptor in the neuromuscular junction. Blocks acetylcholine activity at the postsynaptic membrane. The biotin conjugate can be subsequently labeled with anti-biotin antibodies, avidin, or streptavidin for signal amplification.

A biotin-conjugated cAMP analog that can be used to probe cAMP receptors.

A high affinity inhibitor of the nicotinic acetylcholine receptor in the neuromuscular junction. Blocks acetylcholine activity at the postsynaptic membrane.

Calcein AM is a widely used green fluorescent cell marker. Calcein AM is membrane-permeant and thus can be introduced into cells via incubation.

TRITC-DHPE  is a rhodamine-labeled glycerophosphoethanolamine lipid. DHPE conjugates of rhodamine dyes have been used in membrane fusion assays.

Rhodamine-DHPE is a rhodamine-labeled glycerophosphoethanolamine lipid that has been used in membrane fusion assays.

Texas Red®-DHPE is a rhodamine-labeled glycerophosphoethanolamine lipid.

Fluorescein-DHPE is a useful surface pH indicator, and has also been used for measuring lateral diffusion in membranes.

NBD-PE (N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)-1,2-dihexadecanoyl-snglycero-3-phosphoethanolamine, triethylammonium salt) in combination with Rhodamine-DHPE (#60026) or Texas Red®-DHPE (#60027) has been used to study membrane fusion via fluorescence resonance energy transfer (FRET) (1-3). NBD-PE has also been used in photobleaching recovery measurement.

Biotin-DHPE (N-(Biotinoyl)-1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine, triethylammonium salt) can be used to couple avidin or streptavidin to cell membranes or liposomes.

Biotin-X-DHPE (N-((6-(Biotinoyl)amino)hexanoyl)-1,2-dihexadecanoylsn-glycero-3-phosphoethanolamine, triethylammonium salt) can be used to couple avidin or streptavidin to cell membranes or liposomes.

5-Aminoallyl-UTP can be enzymatically incorporated into RNA for subsequent labeling with an amine-reactive fluorescent dye, biotin, or other hapten via conventional peptide coupling methods.