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Dihydrorhodamine 123

Dihydrorhodamine 123 is the reduced form of rhodamine 123 (70010), which is a commonly used fluorescent mitochondrial dye.

Product Attributes

CAS number


Cellular localization


For live or fixed cells

For live/intact cells

Assay type/options

No-wash staining, Real-time imaging




505/534 nm (end product)

Catalog #
10 mg
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Supporting Documents

Product Description

Dihydrorhodamine 123 is the reduced form of rhodamine 123 (70010), which is a commonly used fluorescent mitochondrial dye. Dihydrorhodamine 123 itself is non-fluorescent, but it readily enters cells and is oxidized by oxidative species or by cellular redox systems to the fluorescent rhodamine 123 that accumulates in mitochondrial membranes (1). Dihydrorhodamine 123 is useful for detecting reactive oxygen species including superoxide (in the presence of peroxidase or cytochrome c) (2,3) and peroxynitrite (4,5). Also see dihydrorhodamine 123 dihydrochloride (10056), a more stable and water soluble form of dihydrorhodamine 123.

  • White solid soluble in DMSO
  • Store at -20°C and protect from air and light, especially when in solution
  • C20H18N2O3
  • MW: 346
  • [109244-58-8]


1. Br J of Pharmacol (2010) doi: 10.1111/j.1476-5381.2010.01120.x

2. J Immunol Meth 178, 89 (1995).

3. Biochemistry 34, 3544 (1995).

4. Eur J Biochem. 217, 973 (1993).

5. Arc Biochem Biophys 302, 348 (1993).


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