Rhodamine 123 is a popular green fluorescent mitochondrial dye that stains mitochondria in living cells in a membrane potential-dependent fashion. It is widely used in flow cytometry studies involving mitochondrial membrane potential.
Rhodamine 123 is a popular green fluorescent mitochondrial dye that stains mitochondria in living cells in a membrane potential-dependent fashion. It is widely used in flow cytometry studies involving mitochondrial membrane potential. Please also see Dihydrorhodamine 123 and its HCl salt (10055 and 10056).
- λEx/λEm (MeOH) = 505/534 nm
- ε (505 nm, MeOH) = 97,000
- Orange red solid soluble in DMSO or DMF
- Store at 4°C and protect from light, especially in solution
- MW: 381
1. Cytometry 17, 50 (1994).
2. Science 218, 1117 (1982).
3. J Cell Biol 88, 526 (1981).
You may also like…
An improved and more soluble formulation of the JC-1 dye used for ratiometric detection of mitochondrial potential changes.
Dihydrorhodamine 123 is the reduced form of rhodamine 123 (70010), which is a commonly used fluorescent mitochondrial dye.
JC-1 Mitochondrial Membrane Potential Detection Kit
JC-1 dye is used for fluorescence-based measurement of mitochondrial membrane potential changes in cells, a key measure of cell viability.
LysoView™ fluorescent dyes label lysosomes in live cells without a wash step, and are available in multiple colors with options for super-resolution imaging.
MitoView™ Mitochondrial Dyes
Fluorogenic mitochondrial stains for live cells that rapidly accumulate in mitochondria and can be imaged without washing.
NucSpot® Live Cell Nuclear Stains
No-wash, fluorescent DNA nuclear stains dyes with low toxicity for long-term live cell imaging or fixed cell staining. Choose green or far-red fluorescence.