Reactive CF® Dyes, Other Reactive Dyes & Biotinylation Reagents

Biotin-XX succinimidyl ester (6-((6-((Biotinoyl)amino)hexanoyl)amino)hexanoic acid succinimidyl ester) is readily coupled to a primary or secondary amine under mild conditions. The longer XX arm facilitates the interaction between biotin and avidin or streptavidin.

Biotin SE (Biotin, succinimidyl ester) is readily coupled to a primary or secondary amine under mild conditions.

NBD fluoride (4-Fluoro-7-nitrobenzo-2-oxa-1,3-diazole) is similar to NBD chloride (90045) except that the former is much more reactive toward amines.

The fluorescence of NBD is highly dependent on the environment; it is virtually nonfluorescent in water but becomes strongly green fluorescent in hydrophobic media. NBD-ethylenediamine can be readily coupled to activated carboxy groups or sulfonyl chlorides.

5(6)-SFX has a 6-atom aminohexanoyl spacer group that separates the fluorescein dye and the amine-reactive group.

NBD chloride (4-Chloro-7-nitrobenzo-2-oxa-1,3-diazole) is nonfluorescent until it reacts with primary or secondary amines to produce a fluorescent product. NBD chloride has been extensively used as a derivatizing reagent for chromatography analysis of amino acids and low molecular weight amines.

5-(and-6)-Carboxy-2′,7′-dichlorofluorescein diacetate, succinimidyl ester is a useful fluorescent tracer that can passively diffuse into cells and covalently label intracellular proteins, resulting in long-term cell labeling. The reagent itself is colorless and nonfluorescent but becomes brightly green fluorescent once it is hydrolyzed by intracellular esterases.

5(6)-CFDA, SE (full name: 5-(and-6)-Carboxyfluorescein diacetate, succinimidyl ester, also known as CFSE) is a useful fluorescent tracer that can passively diffuse into cells and covalently label intracellular proteins, resulting in long-term cell labeling. The reagent itself is colorless and non-fluorescent but becomes brightly green fluorescent once it is hydrolyzed by intracellular esterases.

6-ROX, SE (6-Carboxy-X-rhodamine, succinimidyl ester, single isomer) is the amine-reactive form of 6-carboxy-X-rhodamine single isomer.

Full name: 6-Carboxytetramethylrhodamine succinimidyl ester single isomer. Amine-reactive form ofcarboxytetramethylrhodamine (TAMRA) single isomer.

5-ROX, SE (5-Carboxy-X-rhodamine, succinimidyl ester, single isomer) is the amine-reactive form of 5-carboxy-X-rhodamine single isomer.

Amine-reactive form of 6-carboxyrhodamine 6G single isomer.

Full name: 5-Carboxytetramethylrhodamine succinimidyl ester (single isomer). Amine-reactive form of carboxytetramethylrhodamine (TAMRA) single isomer.

Amine-reactive form of 5-carboxyfluorescein single isomer.

Amine-reactive form of 5-carboxyrhodamine 6G single isomer.

APTS is a very useful green fluorescent dye for labeling glycoproteins or sugar molecules in general. The labeling occurs via reductive amination which involves the formation of a Schiff base between the amine of APTS and the aldehyde or ketone of the sugar, followed by the reduction of the Schiff base linkage to a stable carbon-nitrogen bond.