NucView Caspase-3 Substrates
- Real-time detection of caspase-3/7 activity in intact cells
- Homogenous, no wash 15-30 minute assay, or long-term real-time monitoring of caspase activity
- Fixable nuclear staining
- NucView™ 488: Green fluorescence detection for flow cytometry, microscopy, or microplate reader
- NucView™ 405: Blue fluorescence detection for flow cytometry or microscopy using the 405 nm laser line
- NucView™ 530: Orange fluorescence detection for microscopy or flow cytometry
New video from Essen Bioscience featuring NucView™ 488 Caspase 3 Substrate
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NucView™ Caspase-3 Substrates
In the case of NucView Caspase-3 Substrates, the caspase-3 substrate peptide DEVD is attached to a DNA-binding dye, making the dye unable to bind to DNA and thus non-fluorescent. The substrate enters the cell cytoplasm where it is cleaved by caspase-3 to release the high-affinity fluorogenic DNA dye, which migrates to the cell nucleus to stain the nucleus. Therefore, the bi-functional substrate allows one not only to detect caspase-3 within an apoptotic cell but also to visualize apopototic nuclear morphology. Unlike fluorescently-labeled caspase inhibitor assays (FLICA) that use irreversible inhibitors to label active caspases, NucView Caspase-3 Substrates do not interfere with caspase activity, allowing monitoring of caspase activity in real time.
Now, in addition to our green fluorogenic NucView™ 488 Caspase-3 Substrate, Biotium offers the new blue NucView™ 405 and orange NucView™ 530 fluorogenic caspase-3 substrates. NucView™ 405 dye is excited by the 405 nm laser line for detection by confocal microscopy in the DAPI channel (Figure 2), or by flow cytometry in the Pacific Blue® channel (Figure 3), for multi-color applications in which the green fluorescence channel is reserved for other detection reagents. NucView™ 530 (excitation/emission 528/563 nm) can be detected by microscopy or flow cytometry in the Cy®3 or R-PE channels.
NucView™ Caspase-3 Substrates are available as a stand-alone substrates, or in kits with other fluorescent apoptosis and necrosis probes for studying the temporal and spatial relations among the different events by fluorescence microscopy or flow cytometry. See NucView™ products…