RNA & DNA Isolation from FFPE Tissue Samples
Formalin-fixed tissue samples are a challenge for RNA and DNA extraction, often resulting in low yields and poor performance in subsequent steps. Most existing methods rely on heat to remove crosslinks and adducts, which is only partially effective and leads to additional fragmentation of labile nucleic acids. In contrast, the catalytic CAT5™ technology, built on research initiated at Stanford University, greatly accelerates the removal of formaldehyde damage and allows recovery of higher quality nucleic acids. This results in higher yields, more intact RNA or DNA, and better results in downstream analysis like PCR, microarray, or next-generation sequencing (NGS).
CAT5™ catalytic technology
• Chemical reversal of formaldehyde crosslinking
• Effective: Higher yields compared to other methods
• Gentle: Milder conditions and no harsh solvents
• Higher quality RNA or DNA with less fragmentation
RNAstorm™ Kit for Isolation of RNA from FFPE Tissue Samples
Powered by proprietary CAT5™ technology, the RNAstorm™ FFPE isolation kit enhances the removal of formaldehyde-induced damage and purifies RNA with higher yield and quality, better integrity, and greater amplifiability.
The superior quality of RNAstorm-derived RNA has been demonstrated in many ways, including higher RIN score, higher yield, higher DV200, and improved RNA-seq alignment. According to a tech note from Illumina, the best predictor of successful performance in Illumina RNA-seq is the DV200 score, which represents the percentage of RNA fragments longer than 200 nucleotides. Whether you are performing RNA-seq, RT-qPCR, microarray, or other gene expression analysis, the RNAstorm™ kit is your best chance for success.
DNAstorm™ Kit for Isolation of DNA from FFPE Tissue Samples
Using the same CAT5™ technology as the RNAstorm™ kit, the DNAstorm™ FFPE isolation kit enhances the removal of formaldehyde-induced damage and provides DNA with higher yield, quality and greater amplifiability. The DNAstorm™ kit is the best solution for NGS, qPCR, and other advanced DNA analysis applications.
Peer-reviewed studies demonstrate the RNAstorm™ FFPE RNA Extraction Kit yields high amounts of quality RNA for diverse applications.
Sign up to our e-newsletter to download our Literature Digest: Higher Quality and Yields of FFPE RNA for Diverse Applications with RNAstorm™. Featuring publications that showcase the diverse fields of research and downstream workflows in which RNAstorm™ has been used, including data on improved RNA yields and quality versus other commercially available kits.
RNAstorm™ and DNAstorm™ FFPE Extraction Kits
|CELLDATA RNAstorm™ 2.0 FFPE RNA Extraction Kit
|CELLDATA DNAstorm™ 2.0 FFPE DNA Extraction Kit
|CELLDATA DNAstorm™/RNAstorm™ 2.0 Combination Kit
|CELLDATA DNAstorm™ 2.0 MagBead FFPE DNA Extraction Kit
RNA isolation from fresh tissue and cells
Purify high quality total RNA from either cultured cells or fresh tissue in as little as 20 minutes. High yields (up to 120 μg) can be obtained using a simple and quick column-based protocol. Contaminating DNA is removed using a DNase treatment step, and the protocol avoids toxic chemicals such as phenol or chloroform.
Advantages of the RNAstorm™ RNA Isolation Kit
- High quality total RNA in as little as 20 minutes
- Simple workflow yielding up to 120 μg of RNA
- DNase treatment included
- No phenol-chloroform and no ethanol precipitation
- Ready to use for next-generation sequencing (RNA-Seq), RT-PCR, cDNA synthesis, and microarrays
|CELLDATA RNAstorm™ Fresh Cell and Tissue RNA Isolation Kit
DNA Gel Extraction Kit
Biotium’s DNA Gel Extraction Kit is a silica-gel spin column based DNA extraction kit designed to purify 40 bp-40 kb DNA fragments from agarose gels in TAE or TBE buffer. Each column has a maximum binding capacity of 10 ug DNA. Biotium’s DNA Gel Extraction Kit removes GelRed™ and GelGreen™ Nucleic Acid Gel Stains, along with other commonly used nucleic acid gel stains. The purified DNA is compatible with common downstream applications such as transformation, sequencing, PCR, and restriction digests.
|DNA Gel Extraction Kit