Improve Downstream Results for RNA-Seq, cDNA Synthesis, RT-PCR, and Microarrays
Sensitive & Convenient RNA Evaluation
Evaluate RNA Integrity & DNA Contamination with Less Sample
Staining RNA with ethidium bromide (EtBr) requires a significant amount of the dye yet still offers very limited sensitivity for RNA when prestaining or post-staining gels. EMBER500™ was developed by Biotium scientists to offer much brighter signal and higher sensitivity than EtBr for RNA gel staining, allowing users to use less RNA sample to quickly verify RNA is free from degradation and DNA contamination. For maximum convenience, EMBER500™ RNA Prestain Loading Dye includes formamide as well as electrophoresis tracking dyes for convenient denaturing, loading, tracking, and staining in a single step. The prestain is supplied as a 2X concentration, for convenient loading of sample and dye in equal volumes.
Features and benefits
- Sensitive: Brighter and much more sensitive than EtBr, allows use of less RNA sample
- Convenient: Denature, load, track and stain RNA samples in a single step on a regular agarose gel
- Versatile: Stains both DNA and RNA for evaluating total RNA integrity and DNA contamination
- Flexible Detection: Detect with UV transilluminators or blue LED gel imagers
EMBER500™ RNA Prestain Loading Dye
| Product Name | Catalog Number | Size |
|---|---|---|
| EMBER500™ RNA Prestain Loading Dye | 41032 | 4 x 1 mL |
| EMBER500™ RNA Prestain Loading Dye | 41032-250uL | 250 uL |
See the Difference
ACCURATE RNA QUANTITATION FOR QUBIT® OR MICROPLATE READER
Highly Selective & Linear Fluorescence-Based RNA Quantitation
The AccuBlue® Broad Range RNA Quantitation Kit boasts a wide linear range (5 ng–1000 ng RNA), while providing exceptional accuracy, sensitivity, and RNA selectivity. It is ideal for use in quantifying RNA for sensitive applications such as Next-Gen Sequencing (NGS) or reverse transcription PCR (RT-PCR). Unlike absorbance-based measurements, AccuBlue® RNA Quantitation Kit is highly selective for single-stranded RNA over double-stranded DNA, and can tolerate an equal amount of dsDNA in the sample without significant effect on RNA quantitation (purified RNA samples are still recommended). The assay is also able to accurately quantify short ssRNA such as miRNA, while showing very low signal with double-stranded RNA. A mammalian RNA standard is included for accurate quantitation of mammalian cell RNA.
Features and benefits
- Selective: Can tolerate equivalent amounts of dsDNA (see Figure 2 below)
- Convenient: Wide linear range spans Qubit®/Quant-iT™ HS and BR ranges
- Flexible: For fluorescence microplate reader or small fluorometer like Qubit®
- Accurate: The mammalian-derived total RNA standard provides the highest accuracy when quantifying samples from similar sources
Exceptional Linearity
Highly Selective for ssRNA
AccuBlue® Broad Range RNA Quantitation Kits
| Product | Catalog No. | Size |
|---|---|---|
| AccuBlue® Broad Range RNA Quantitation Kit | 31073-T | Trial size (200 assays) |
| AccuBlue® Broad Range RNA Quantitation Kit | 31073 | 1000 assays |
Extract More Amplifiable RNA from FFPE Tissues
Tired of Poor Results with RNA from FFPE Tissues?
FFPE samples or other formalin-fixed tissue are a challenge for RNA extraction, often resulting in low yields and poor performance for downstream applications such as RT-PCR and next-generation sequencing (NGS). Most existing extraction methods rely on high heat to remove crosslinks and adducts, which are only partially effective and lead to additional fragmentation.
Try RNAstorm™ 2.0 FFPE RNA Extraction Kit
The CELLDATA RNAstorm™ 2.0 FFPE RNA Extraction Kit uses proprietary CAT5™ catalytic technology, built on research initiated at Stanford University, to greatly accelerate the removal of formaldehyde damage without excessive heat or harsh solvents. This allows greater yields of amplifiable RNA which leads to better results in downstream analysis like RT-PCR, microarray, or NGS. The superior quality of RNAstorm™-derived RNA has been demonstrated by a higher RIN score, higher yield, higher DV200, and improved RNA-seq alignment. According to a tech note from Illumina, the best predictor of successful performance in Illumina RNA-seq is the DV200 score, which represents the percentage of RNA fragments longer than 200 nucleotides. Whether you are performing RNA-seq, RT-qPCR, microarray, or other gene expression analysis, the RNAstorm™ 2.0 kit is your best chance for success.
Features and benefits
- Novel CAT5™ technology: Proprietary method for chemical reversal of formaldehyde crosslinking
- Higher Yields: Offers higher yields of RNA over competitor FFPE extraction kits
- Greater Amplifiability: Yields RNA with higher integrity and less fragmentation for improved downstream analysis
- Gentle: Milder conditions that don’t require high temperatures or harsh solvents
- Simple: Easy to follow spin column-based workflow
CELLDATA RNAstorm™ FFPE RNA Extraction Kits
| Product Name | Catalog Number | Size |
|---|---|---|
| CELLDATA RNAstorm™ 2.0 FFPE RNA Extraction Kit | CD506 | 50 preps |
Peer-reviewed studies demonstrate the RNAstorm™ FFPE RNA Extraction Kit yields high amounts of quality RNA for diverse applications.
Sign up to our e-newsletter to download our Literature Digest: Higher Quality and Yields of FFPE RNA for Diverse Applications with RNAstorm™. Featuring publications that showcase the diverse fields of research and downstream workflows in which RNAstorm™ has been used, including data on improved RNA yields and quality versus other commercially available kits.
High Quality RNA from Fresh Cells & Tissues in Minutes
RNAstorm™ Kit for RNA Isolation from Fresh Cells and Tissues
The CELLDATA RNAstorm™ Fresh Cell and Tissue RNA Isolation Kit allows extraction of high quality total RNA from cultured cells or fresh tissue in as little as 20 minutes. High yields (up to 120 μg) can be obtained using a simple and quick column-based protocol. Contaminating DNA is removed using a DNase treatment step, and the protocol avoids toxic chemicals such as phenol or chloroform.
Features and benefits
- High Yield: Yields up to 120 μg of total RNA
- Rapid & Simple: 20 minute spin-column workflow, no phenol-chloroform or precipitation
- Versatile Downstream Analysis: High quality RNA for RNA-Seq, RT-PCR, cDNA synthesis, or microarrays
| Product Name | Catalog Number | Size |
|---|---|---|
| CELLDATA RNAstorm™ Fresh Cell and Tissue RNA Isolation Kit | CD504 | 50 preps |
EV TOTAL RNA EXTRACTION
Efficient total RNA extraction from purified EVs
The ExoBrite™ EV Total RNA Isolation Kit was designed to address the challenges of EV RNA extraction by offering an optimized and easy-to-use kit for total RNA isolation, including mRNA and miRNAs, from purified EVs. The isolated EV RNA can then be used for downstream analysis such as qPCR or RNAseq.
Features and benefits
- Developed for EVs: Optimized for total RNA extraction from purified EVs
- High-Yields: Recover ~10-20 ng of RNA from 1×1010 SEC-enriched EVs
- Downstream Analysis: Suitable for applications such as qPCR or RNAseq
- Easy: Simple column-based purification with no phenol/chloroform or ethanol precipitation steps
| Product Name | Catalog Number | Size |
|---|---|---|
| ExoBrite™ EV Total RNA Isolation Kit | 28001 | 50 preps |
Eliminate RNase Activity with RNAse-X™
Protect Precious RNA Samples with RNase-X™
RNase-X™ Decontamination Solution is a ready-to-use cleaning agent for effective removal of RNase and other contaminants from working surfaces. The solution features an optimized formulation of key ingredients for maximum removal of RNase to protect precious RNA samples during purification, quantitation, amplification, and other analytical workflows. The solution may be applied to benchtops, pipets, tip boxes, and instruments as well as the outside of reaction vessels such as microfuge tubes. RNase-X™ is provided in a convenient spray bottle and performs as well as RNaseZap™ and other common decontamination reagents.
Features and benefits
- High-Performance: Effectively eliminates RNase contamination on work surfaces
- Versatile: Ideal for cleaning benchtops, pipettors, and other tools
- Convenient: Provided in a ready-to-use spray bottle
- Cost-Effective: Excellent performance at a lower cost compared to RNaseZap™
RNase-X™ Decontamination Solution
| Product Name | Catalog Number | Size |
|---|---|---|
| RNase-X™ Decontamination Solution | 22028 | 250 mL |
Sensitive Detection of RNase Activity
Ideal for QC Workflows or Detecting Contamination
Reliably detect RNase activity in liquid samples with the RNaseReveal™ Activity Assay Kit. Featuring a highly optimized buffer and substrate for maximum sensitivity, the RNaseReveal™ Activity Assay Kit is an ideal solution for control quality workflows or for detecting RNase contamination in solutions to be used with RNA work.
The substrate can be detected by any fluorescence plate reader or fluorometer capable of detecting green fluorescence. Biotium also offers RNase A (Cat. No. 99871-600uL) for use with this kit.
Features and benefits
- Reliable RNase Detection: Highly optimized buffer and substrate for maximum sensitivity
- Rapid: Simple 40 minute assay
- Versatile: Suitable for handheld fluorometer or fluorescence plate reader
RNaseReveal™ Activity Assay Kit
| Product Name | Catalog Number | Size |
|---|---|---|
| RNaseReveal™ Activity Assay Kit | 31086 | 100 assays |