GelRed & GelGreen nucleic acid gel stains
Environmentally safe and ultra-sensitive nucleic acid gel stains for replacing EtBr
- Safer than EtBr
Shown by Ames test and other tests to be nonmutagenic and noncytotoxic.
- Easy disposal
Passed environmental safety tests for direct disposal down the drain or in regular trash.
Much more sensitive than EtBr and SYBR® Safe.
- Extremely stable
Stable at room temperature for long-term storage.
- Flexible for different procedures
Use in either precast or post electrophoresis gel staining, compatible with common downstream applications like cloning and sequencing
- Compatible with standard UV transilluminator or visible light gel reader
GelRed™ replaces EtBr with no optical setting change; GelGreen replaces SYBR® dyes with no optical setting change
- NEW! PAGE GelRed™ and PAGE GelGreen™ for staining DNA in polyacrylamide gels
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GelRed™ Is Superior to EtBr
GelGreen Is Unmatched by SYBR® Safe
EtBr has been the predominant dye used for nucleic acid gel staining for decades because of its low price and generally sufficient sensitivity. However, EtBr is a highly mutagenic material. The safety hazard and costs associated with decontamination and waste disposal can ultimately make the dye expensive to use. For this reason, alternative gel stains, such as SYBR® dyes, have become commercially available in recent years. Although these alternative dyes have reduced mutagenicity, they often have to sacrifice other aspects of the dyes.
For example, SYBR® Safe has very limited sensitivity while SYBR® Green and SYBR® Gold are much less stable than EtBr. SYBR dyes also enter cells rapidly to stain mitochondria and nuclear DNA, making it more likely for the dyes to be toxic at high enough concentrations. Indeed, SYBR® Green I is known to strongly potentiate mutation caused by UV light and another mutagens (Ohta, et al. Mutat. Res. 492, 91(2001)).
To make GelRed™ and GelGreen™ safe, scientists at Biotium used a novel yet very simple concept: reducing genotoxicity by preventing the dyes from entering living cells. We believe that a DNA-binding dye can be made nonmutagenic or substantially so by denying its chance to be in contact with genomic DNA in living cells. Thus, we engineered the chemical structures of GelRed™ and GelGreen™ such that the dyes are incapable of crossing cell membranes. The Ames test confirmed that GelRed™ and GelGreen™ are nonmutagenic at concentrations well above their working concentrations used for gel staining. Furthermore, environmental safety tests showed that GelRed™ and GelGreen™ are nonhazardous and nontoxic to aquatic life. As a result, GelRed™ and GelGreen™ can be disposed of down the drain or in regular trash. For more information, please download the GelRed™/GelGreen™ Safety Report.
GelRed™ and GelGreen™ are highly sensitive either as precast gel stains or post gel stains. GelRed™ is much more sensitive than EtBr, and at least as sensitive as or brighter than SYBR Gold in post gel staining. Unlike SYBR Gold, GelRed™ can also be used as a highly sensitive precast gel stain. GelGreen™ is spectrally similar to SYBR® Safe, but is far more sensitive than the latter, and can be used with blue light gel imaging systems like the DarkReader®.
Another major advantage of GelRed™ and GelGreen™ is their remarkable stability. You can store and handle the two dyes the same way you do with EtBr.
GelGreen™ and GelRed™ and their uses are covered by US patent numbers 7960498, 7803943, and 8232050.
SYBR is a registered trademark of Invitrogen Corp; DarkReader is a registered trademark of Clare Chemical.